An ultra-simplified protocol for PCR template preparation from both unsporulated and sporulated Eimeria oocysts
Molecular biological techniques have enabled the accurate identification of the avian Eimeria parasite, however, the preparation of PCR template remains a bottleneck due to contaminants from feces and the robust oocyst's wall resistant to chemical and mechanical force. Generally, the preparatio...
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Main Authors: | Aruto Takano, Dennis V. Umali, April H. Wardhana, Dyah H. Sawitri, Isao Teramoto, Toshimitsu Hatabu, Yasutoshi Kido, Akira Kaneko, Kazumi Sasai, Hiromitsu Katoh, Makoto Matsubayashi |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-03-01
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Series: | Poultry Science |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000471 |
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