An ultra-simplified protocol for PCR template preparation from both unsporulated and sporulated Eimeria oocysts

An ultra-simplified protocol for PCR template preparation from both unsporulated and sporulated Eimeria oocysts

Molecular biological techniques have enabled the accurate identification of the avian Eimeria parasite, however, the preparation of PCR template remains a bottleneck due to contaminants from feces and the robust oocyst's wall resistant to chemical and mechanical force. Generally, the preparatio...

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Bibliographic Details
Main Authors: Aruto Takano, Dennis V. Umali, April H. Wardhana, Dyah H. Sawitri, Isao Teramoto, Toshimitsu Hatabu, Yasutoshi Kido, Akira Kaneko, Kazumi Sasai, Hiromitsu Katoh, Makoto Matsubayashi
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Poultry Science
Subjects:
Coccidian parasite
Eimeria tenella
Extraction
Molecular identification
Oocyst
Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125000471
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