Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes
Summary: Mouse oocytes devoid of branched actin display prolonged cortical contractions during the first meiotic division. Here, we present a protocol to collect fully grown oocytes in prophase I arrest and record their contractions during the first meiotic division through time-lapse imaging in tra...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000103 |
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| author | Gaëlle Letort Elvira Nikalayevich |
| author_facet | Gaëlle Letort Elvira Nikalayevich |
| author_sort | Gaëlle Letort |
| collection | DOAJ |
| description | Summary: Mouse oocytes devoid of branched actin display prolonged cortical contractions during the first meiotic division. Here, we present a protocol to collect fully grown oocytes in prophase I arrest and record their contractions during the first meiotic division through time-lapse imaging in transillumination, without additional labeling. We then explain how to set up the pipeline to segment the oocyte outline with an ImageJ plugin Oocytor and to measure cortical fluctuations with the help of an ImageJ plugin Radioak.For complete details on the use and execution of this protocol, please refer to Nikalayevich et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-f4ffb78555d14d8ba5c69ed773cf0641 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-f4ffb78555d14d8ba5c69ed773cf06412025-01-31T05:12:25ZengElsevierSTAR Protocols2666-16672025-03-0161103604Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytesGaëlle Letort0Elvira Nikalayevich1Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, Université Paris Cité, 25 Rue du Dr. Roux, 75015 Paris, France; Corresponding authorCenter for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, 75005 Paris, France; Corresponding authorSummary: Mouse oocytes devoid of branched actin display prolonged cortical contractions during the first meiotic division. Here, we present a protocol to collect fully grown oocytes in prophase I arrest and record their contractions during the first meiotic division through time-lapse imaging in transillumination, without additional labeling. We then explain how to set up the pipeline to segment the oocyte outline with an ImageJ plugin Oocytor and to measure cortical fluctuations with the help of an ImageJ plugin Radioak.For complete details on the use and execution of this protocol, please refer to Nikalayevich et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000103cell culturesingle celldevelopmental biologymicroscopy |
| spellingShingle | Gaëlle Letort Elvira Nikalayevich Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes STAR Protocols cell culture single cell developmental biology microscopy |
| title | Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes |
| title_full | Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes |
| title_fullStr | Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes |
| title_full_unstemmed | Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes |
| title_short | Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes |
| title_sort | protocol to image segment and quantify cortical contractions in maturing mouse oocytes |
| topic | cell culture single cell developmental biology microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000103 |
| work_keys_str_mv | AT gaelleletort protocoltoimagesegmentandquantifycorticalcontractionsinmaturingmouseoocytes AT elviranikalayevich protocoltoimagesegmentandquantifycorticalcontractionsinmaturingmouseoocytes |