Protocol to image, segment, and quantify cortical contractions in maturing mouse oocytes

Summary: Mouse oocytes devoid of branched actin display prolonged cortical contractions during the first meiotic division. Here, we present a protocol to collect fully grown oocytes in prophase I arrest and record their contractions during the first meiotic division through time-lapse imaging in tra...

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Bibliographic Details
Main Authors: Gaëlle Letort, Elvira Nikalayevich
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000103
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