Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues

Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues

Abstract Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of the staining system, we developed a highly optimized 3D staining imaging pipeline based on CUBIC. Based on our precise characteri...

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Main Authors: Etsuo A. Susaki, Chika Shimizu, Akihiro Kuno, Kazuki Tainaka, Xiang Li, Kengo Nishi, Ken Morishima, Hiroaki Ono, Koji L. Ode, Yuki Saeki, Kazunari Miyamichi, Kaoru Isa, Chihiro Yokoyama, Hiroki Kitaura, Masako Ikemura, Tetsuo Ushiku, Yoshihiro Shimizu, Takashi Saito, Takaomi C. Saido, Masashi Fukayama, Hirotaka Onoe, Kazushige Touhara, Tadashi Isa, Akiyoshi Kakita, Mitsuhiro Shibayama, Hiroki R. Ueda
Format: Article
Language:English
Published: Nature Portfolio 2020-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-15906-5
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https://doi.org/10.1038/s41467-020-15906-5

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