Changes of anthocyanin, sugar and acid accumulation and expression characteristics of related metabolic genes in Tarocco blood oranges during room temperature storage
Using Tarocco blood oranges as experimental materials, the changes of anthocyanin, soluble sugar and organic acid contents and the expression characteristics involved in related metabolic genes during room temperature storage were analyzed by high performance liquid chromatography (HPLC) and quantit...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2021-10-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.01.041 |
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| Summary: | Using Tarocco blood oranges as experimental materials, the changes of anthocyanin, soluble sugar and organic acid contents and the expression characteristics involved in related metabolic genes during room temperature storage were analyzed by high performance liquid chromatography (HPLC) and quantitative real-time polymerase chain reaction. The results showed as follows: During room temperature storage, the anthocyanin content of blood oranges was increased by 222%. The mRNA levels of 4CL, CHI and ANS were significantly positive correlation with the anthocyanin content. The total sugar, sucrose, fructose and glucose contents of the fruits were significantly promoted by 12.0%, 7.7%, 19.7% and 12.7%, respectively. The mRNA level of SS1 was significantly positive correlation with the sucrose content, and the mRNA level of AI was extremely significant and negative correlation with the sucrose content. The accumulation of citric acid fluctuated significantly, and the mRNA level of IDH was extremely significant and negative correlation with the citric acid content. The 4CL, CHI, ANS, SS1, AI and IDH genes played important roles in the metabolism of anthocyanin, sugar and acid. Correlation analysis indicated that the anthocyanin content in blood oranges was significantly positive correlation with the contents of total sugar, sucrose, fructose, and glucose during room temperature storage, which had the highest correlation with the sucrose content. The above results show that the anthocyanin and soluble sugar contents of Tarocco blood oranges are promoted by short-term room temperature storage, and the changes of soluble sugar accumulation may regulate the anthocyanin synthesis. |
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| ISSN: | 1008-9209 2097-5155 |