Influence of chemical concentrations on the physicochemical, structural, functional and color characteristics of chitin isolated from Arabian red shrimp (Aristeus alcocki)

This study examines the physicochemical, functional and structural characteristics of α-chitin extracted from the exoskeleton of Arabian red shrimp using varying concentrations of HCl and NaOH. By employing HCl and NaOH at concentrations of 0.6, 1.0 and 1.4 M, this study systematically evaluates the...

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Main Authors: M.K. Rasweefali, A. Nayana, M.K. Raseel Rahman, H. Habeebrehman, S. Sabu
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Sustainable Chemistry for the Environment
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Online Access:http://www.sciencedirect.com/science/article/pii/S2949839225000288
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author M.K. Rasweefali
A. Nayana
M.K. Raseel Rahman
H. Habeebrehman
S. Sabu
author_facet M.K. Rasweefali
A. Nayana
M.K. Raseel Rahman
H. Habeebrehman
S. Sabu
author_sort M.K. Rasweefali
collection DOAJ
description This study examines the physicochemical, functional and structural characteristics of α-chitin extracted from the exoskeleton of Arabian red shrimp using varying concentrations of HCl and NaOH. By employing HCl and NaOH at concentrations of 0.6, 1.0 and 1.4 M, this study systematically evaluates the degree of acetylation (DA), molecular weight (Mw), whiteness, purity, crystallinity, and other key parameters of the isolated chitin samples in comparison to commercial chitin (CCN). Results indicate a varied range of N-acetyl group content (77.34 ± 1.13 % to 96.56 ± 1.18 %) and crystallinity values (36.40–44.20 %) across different acid and alkali concentration combinations. This study highlights the effectiveness of deproteinization and demineralization processes in most cases, as confirmed by EDS microanalysis, which demonstrated the complete removal of minerals. Morphological analysis using FE-SEM revealed a smooth, porous and microfibrillar structure in the isolated chitin samples. The study emphasizes the significant influence of the extraction process on chitin properties, with lower concentrations of HCl and NaOH yielding higher CrI and DA. Furthermore, increased concentrations of HCl and NaOH were associated with decreased Mw and increased fat-binding capacity (FBC) and water-binding capacity (WBC) of the isolated chitin samples. This study enhances understanding of how species-specific traits and processing conditions influence chitin characteristics, enabling researchers to predict and select crustacean species for chitin isolation based on desired functional properties.
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spelling doaj-art-fd1f38852ef640d0863c3d0096bda1832025-08-20T03:10:21ZengElsevierSustainable Chemistry for the Environment2949-83922025-06-011010023310.1016/j.scenv.2025.100233Influence of chemical concentrations on the physicochemical, structural, functional and color characteristics of chitin isolated from Arabian red shrimp (Aristeus alcocki)M.K. Rasweefali0A. Nayana1M.K. Raseel Rahman2H. Habeebrehman3S. Sabu4School of Industrial Fisheries, Cochin University of Science and Technology, Lakeside Campus, Cochin, Kerala 682016, India; Corresponding authors.School of Industrial Fisheries, Cochin University of Science and Technology, Lakeside Campus, Cochin, Kerala 682016, IndiaDepartment of Physics, SAFI Institute of Advanced Study (Autonomous), Malappuram, Kerala 673633, IndiaPG and Research Department of Zoology, Farook College, Kozhikode, Kerala 673632, IndiaSchool of Industrial Fisheries, Cochin University of Science and Technology, Lakeside Campus, Cochin, Kerala 682016, India; Corresponding authors.This study examines the physicochemical, functional and structural characteristics of α-chitin extracted from the exoskeleton of Arabian red shrimp using varying concentrations of HCl and NaOH. By employing HCl and NaOH at concentrations of 0.6, 1.0 and 1.4 M, this study systematically evaluates the degree of acetylation (DA), molecular weight (Mw), whiteness, purity, crystallinity, and other key parameters of the isolated chitin samples in comparison to commercial chitin (CCN). Results indicate a varied range of N-acetyl group content (77.34 ± 1.13 % to 96.56 ± 1.18 %) and crystallinity values (36.40–44.20 %) across different acid and alkali concentration combinations. This study highlights the effectiveness of deproteinization and demineralization processes in most cases, as confirmed by EDS microanalysis, which demonstrated the complete removal of minerals. Morphological analysis using FE-SEM revealed a smooth, porous and microfibrillar structure in the isolated chitin samples. The study emphasizes the significant influence of the extraction process on chitin properties, with lower concentrations of HCl and NaOH yielding higher CrI and DA. Furthermore, increased concentrations of HCl and NaOH were associated with decreased Mw and increased fat-binding capacity (FBC) and water-binding capacity (WBC) of the isolated chitin samples. This study enhances understanding of how species-specific traits and processing conditions influence chitin characteristics, enabling researchers to predict and select crustacean species for chitin isolation based on desired functional properties.http://www.sciencedirect.com/science/article/pii/S2949839225000288Deep-sea shrimpChitin preparationCharacterizationMolecular weightDegree of acetylationChemical treatment
spellingShingle M.K. Rasweefali
A. Nayana
M.K. Raseel Rahman
H. Habeebrehman
S. Sabu
Influence of chemical concentrations on the physicochemical, structural, functional and color characteristics of chitin isolated from Arabian red shrimp (Aristeus alcocki)
Sustainable Chemistry for the Environment
Deep-sea shrimp
Chitin preparation
Characterization
Molecular weight
Degree of acetylation
Chemical treatment
title Influence of chemical concentrations on the physicochemical, structural, functional and color characteristics of chitin isolated from Arabian red shrimp (Aristeus alcocki)
title_full Influence of chemical concentrations on the physicochemical, structural, functional and color characteristics of chitin isolated from Arabian red shrimp (Aristeus alcocki)
title_fullStr Influence of chemical concentrations on the physicochemical, structural, functional and color characteristics of chitin isolated from Arabian red shrimp (Aristeus alcocki)
title_full_unstemmed Influence of chemical concentrations on the physicochemical, structural, functional and color characteristics of chitin isolated from Arabian red shrimp (Aristeus alcocki)
title_short Influence of chemical concentrations on the physicochemical, structural, functional and color characteristics of chitin isolated from Arabian red shrimp (Aristeus alcocki)
title_sort influence of chemical concentrations on the physicochemical structural functional and color characteristics of chitin isolated from arabian red shrimp aristeus alcocki
topic Deep-sea shrimp
Chitin preparation
Characterization
Molecular weight
Degree of acetylation
Chemical treatment
url http://www.sciencedirect.com/science/article/pii/S2949839225000288
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