Double step screening using endogenous marker improves relative gene targeting efficiency in Arabidopsis

Abstract Gene targeting (GT) is a powerful tool for manipulating endogenous genomic sequences as intended. However, its efficiency is rather low, especially in seed plants. Numerous attempts have been made to improve the efficiency of GT via the CRISPR/Cas systems in plants, but these have not been...

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Bibliographic Details
Main Authors: Yiqiu Cheng, Lei Zhang, Yongping Ke, Xiaofei Dang, Daisuke Miki
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-80352-y
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