Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions
Considering the four characteristics of strains, including acid production, acid tolerance, salt tolerance, and nitrite degradation rate, <i>Pediococcus pentosaceus</i> NCU006063 was selected as the fermentation agent, and the medium composition of <i>Pediococcus pentosaceus</i&...
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2025-01-01
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| author | Hong Xu Danyang Li Xue Jiang Qi Pei Zhengqin Li Philippe Madjirebaye Mingyong Xie Tao Xiong Zhanggen Liu |
| author_facet | Hong Xu Danyang Li Xue Jiang Qi Pei Zhengqin Li Philippe Madjirebaye Mingyong Xie Tao Xiong Zhanggen Liu |
| author_sort | Hong Xu |
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| description | Considering the four characteristics of strains, including acid production, acid tolerance, salt tolerance, and nitrite degradation rate, <i>Pediococcus pentosaceus</i> NCU006063 was selected as the fermentation agent, and the medium composition of <i>Pediococcus pentosaceus</i> NCU006063 was optimized using Plackett–Burman and central composite rotational design. Three of the seven factors studied in the Plackett–Burman design significantly affected the viable counts. A central composite rotational design was used to optimize the significant factors and generate response surface plots. Using these response surface plots and point predictions, the optimal factors were soy peptone (38.75 g/L), FeSO<sub>4</sub> (0.10 g/L), and VB<sub>7</sub> (20 g/L). In addition, the optimized incubation conditions were a temperature of 39 °C, an initial pH value of 7, and an inoculation volume of 3%. The optimized biomass production parameters were a constant pH (6.5), neutralizing agent types (25% NH<sub>3</sub>·H<sub>2</sub>O), and gas types (N<sub>2</sub>). Under these optimal conditions, <i>Pediococcus pentosaceus</i> NCU006063 exhibited a great viable bacterial count of up to 2.65 × 10<sup>10</sup> CFU/mL, which is 9.71 times higher than that of MRS broth (2.73 × 10<sup>9</sup> CFU/mL). These results demonstrated that the <i>Pediococcus pentosaceus</i> NCU006063 strain has excellent potential as a fermentation agent and can provide a theoretical base for the in-depth exploration and promotion of fermented cowpea use in human diets. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Foods |
| spelling | doaj-art-fbb13cc0c89549b296aad103894d15242025-01-24T13:32:39ZengMDPI AGFoods2304-81582025-01-0114215010.3390/foods14020150Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production ConditionsHong Xu0Danyang Li1Xue Jiang2Qi Pei3Zhengqin Li4Philippe Madjirebaye5Mingyong Xie6Tao Xiong7Zhanggen Liu8State Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaState Key Laboratory of Food Science and Resources, No. 235 Nanjing East Road, Nanchang 330047, ChinaConsidering the four characteristics of strains, including acid production, acid tolerance, salt tolerance, and nitrite degradation rate, <i>Pediococcus pentosaceus</i> NCU006063 was selected as the fermentation agent, and the medium composition of <i>Pediococcus pentosaceus</i> NCU006063 was optimized using Plackett–Burman and central composite rotational design. Three of the seven factors studied in the Plackett–Burman design significantly affected the viable counts. A central composite rotational design was used to optimize the significant factors and generate response surface plots. Using these response surface plots and point predictions, the optimal factors were soy peptone (38.75 g/L), FeSO<sub>4</sub> (0.10 g/L), and VB<sub>7</sub> (20 g/L). In addition, the optimized incubation conditions were a temperature of 39 °C, an initial pH value of 7, and an inoculation volume of 3%. The optimized biomass production parameters were a constant pH (6.5), neutralizing agent types (25% NH<sub>3</sub>·H<sub>2</sub>O), and gas types (N<sub>2</sub>). Under these optimal conditions, <i>Pediococcus pentosaceus</i> NCU006063 exhibited a great viable bacterial count of up to 2.65 × 10<sup>10</sup> CFU/mL, which is 9.71 times higher than that of MRS broth (2.73 × 10<sup>9</sup> CFU/mL). These results demonstrated that the <i>Pediococcus pentosaceus</i> NCU006063 strain has excellent potential as a fermentation agent and can provide a theoretical base for the in-depth exploration and promotion of fermented cowpea use in human diets.https://www.mdpi.com/2304-8158/14/2/150<i>Pediococcus pentosaceus</i>screening lactic acid bacteriaperformance optimizationbiomass production |
| spellingShingle | Hong Xu Danyang Li Xue Jiang Qi Pei Zhengqin Li Philippe Madjirebaye Mingyong Xie Tao Xiong Zhanggen Liu Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions Foods <i>Pediococcus pentosaceus</i> screening lactic acid bacteria performance optimization biomass production |
| title | Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions |
| title_full | Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions |
| title_fullStr | Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions |
| title_full_unstemmed | Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions |
| title_short | Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions |
| title_sort | screening of lactic acid bacteria isolated from fermented cowpea and optimization of biomass production conditions |
| topic | <i>Pediococcus pentosaceus</i> screening lactic acid bacteria performance optimization biomass production |
| url | https://www.mdpi.com/2304-8158/14/2/150 |
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