Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso)
ABSTRACT: Background: Bacteriological diagnosis of Campylobacter spp, Salmonella spp and Shigella spp could be necessary in the case of infectious gastroenteritis syndrome. The objective of this study was to diagnose concomitantly the three enteropathogenic bacteria by multiplex Real-Time PCR in s...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Knowledge E
2017-09-01
|
| Series: | Sudan Journal of Medical Sciences |
| Online Access: | https://knepublishing.com/index.php/SJMS/article/view/931 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589672246673408 |
|---|---|
| author | Salfo Sawadogo Birama Diarra Cyrille BIsseye Tegwindé Rebeca Compaore Florencia W. Djigma Djénéba Ouermi Aboubakar S. Ouattara Jacques Simpore |
| author_facet | Salfo Sawadogo Birama Diarra Cyrille BIsseye Tegwindé Rebeca Compaore Florencia W. Djigma Djénéba Ouermi Aboubakar S. Ouattara Jacques Simpore |
| author_sort | Salfo Sawadogo |
| collection | DOAJ |
| description | ABSTRACT:
Background: Bacteriological diagnosis of Campylobacter spp, Salmonella spp and Shigella spp could be necessary in the case of infectious gastroenteritis syndrome.
The objective of this study was to diagnose concomitantly the three enteropathogenic bacteria by multiplex Real-Time PCR in stool culture samples in Ouagadougou (Burkina Faso).
Materials and Methods: The study was conducted from February 5th to March 9th, 2013. Two hundred stool samples were received during the study period. The bacteria were identified by bacterial culture following by multiplex Real-Time PCR.
Results: Shigella spp and Campylobacter spp were sought by culture in all 200 samples. Enteropathogenic E. coli was sought only in 37 samples from all children under 2 years old. The bacterial culture was positive in 12 stool samples. Shigella spp and Salmonella spp. were isolated respectively in 5 (2.5%) and 3 samples (1.5%). Enteropathogenic E. coli was isolated in 10.8% (4/37) of the samples tested.
The multiplex real-time PCR identified bacteria in 20 patients, including 17 cases of Shigella spp., 1 case of Salmonella spp. and 2 cases of Campylobacter spp.
Conclusions: This study has highlighted the low frequency of 3 sought bacterial genera in stool samples. It has also demonstrated a significant difference between the culture and the multiplex Real-Time PCR method in the diagnosis of Shigella. |
| format | Article |
| id | doaj-art-f9ef3aceb9b741fe94ffc68a459a0606 |
| institution | Kabale University |
| issn | 1858-5051 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | Knowledge E |
| record_format | Article |
| series | Sudan Journal of Medical Sciences |
| spelling | doaj-art-f9ef3aceb9b741fe94ffc68a459a06062025-01-24T11:20:02ZengKnowledge ESudan Journal of Medical Sciences1858-50512017-09-0112316317310.18502/sjms.v12i3.931931Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso)Salfo Sawadogo0Birama Diarra1Cyrille BIsseye2Tegwindé Rebeca Compaore3Florencia W. Djigma4Djénéba Ouermi5Aboubakar S. Ouattara6Jacques Simpore7Pietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina FasoPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina FasoUniversity of Science and Technique of MasukuPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina FasoPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina FasoPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina FasoCRSBAN, University of Ouaga I Pr Joseph KI-ZERBO, 03 BP 7131 Ouagadougou 03, Burkina FasoPietro Annigoni Biomolecular Research Centre, CERBA/LABIOGENE, University of Ouaga I Pr Joseph KI-ZERBO, 01 BP 364 Ouagadougou 01, Burkina FasoABSTRACT: Background: Bacteriological diagnosis of Campylobacter spp, Salmonella spp and Shigella spp could be necessary in the case of infectious gastroenteritis syndrome. The objective of this study was to diagnose concomitantly the three enteropathogenic bacteria by multiplex Real-Time PCR in stool culture samples in Ouagadougou (Burkina Faso). Materials and Methods: The study was conducted from February 5th to March 9th, 2013. Two hundred stool samples were received during the study period. The bacteria were identified by bacterial culture following by multiplex Real-Time PCR. Results: Shigella spp and Campylobacter spp were sought by culture in all 200 samples. Enteropathogenic E. coli was sought only in 37 samples from all children under 2 years old. The bacterial culture was positive in 12 stool samples. Shigella spp and Salmonella spp. were isolated respectively in 5 (2.5%) and 3 samples (1.5%). Enteropathogenic E. coli was isolated in 10.8% (4/37) of the samples tested. The multiplex real-time PCR identified bacteria in 20 patients, including 17 cases of Shigella spp., 1 case of Salmonella spp. and 2 cases of Campylobacter spp. Conclusions: This study has highlighted the low frequency of 3 sought bacterial genera in stool samples. It has also demonstrated a significant difference between the culture and the multiplex Real-Time PCR method in the diagnosis of Shigella.https://knepublishing.com/index.php/SJMS/article/view/931 |
| spellingShingle | Salfo Sawadogo Birama Diarra Cyrille BIsseye Tegwindé Rebeca Compaore Florencia W. Djigma Djénéba Ouermi Aboubakar S. Ouattara Jacques Simpore Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso) Sudan Journal of Medical Sciences |
| title | Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso) |
| title_full | Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso) |
| title_fullStr | Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso) |
| title_full_unstemmed | Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso) |
| title_short | Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso) |
| title_sort | molecular diagnosis of shigella salmonella and campylobacter by multiplex real time pcr in stool culture samples in ouagadougou burkina faso |
| url | https://knepublishing.com/index.php/SJMS/article/view/931 |
| work_keys_str_mv | AT salfosawadogo moleculardiagnosisofshigellasalmonellaandcampylobacterbymultiplexrealtimepcrinstoolculturesamplesinouagadougouburkinafaso AT biramadiarra moleculardiagnosisofshigellasalmonellaandcampylobacterbymultiplexrealtimepcrinstoolculturesamplesinouagadougouburkinafaso AT cyrillebisseye moleculardiagnosisofshigellasalmonellaandcampylobacterbymultiplexrealtimepcrinstoolculturesamplesinouagadougouburkinafaso AT tegwinderebecacompaore moleculardiagnosisofshigellasalmonellaandcampylobacterbymultiplexrealtimepcrinstoolculturesamplesinouagadougouburkinafaso AT florenciawdjigma moleculardiagnosisofshigellasalmonellaandcampylobacterbymultiplexrealtimepcrinstoolculturesamplesinouagadougouburkinafaso AT djenebaouermi moleculardiagnosisofshigellasalmonellaandcampylobacterbymultiplexrealtimepcrinstoolculturesamplesinouagadougouburkinafaso AT aboubakarsouattara moleculardiagnosisofshigellasalmonellaandcampylobacterbymultiplexrealtimepcrinstoolculturesamplesinouagadougouburkinafaso AT jacquessimpore moleculardiagnosisofshigellasalmonellaandcampylobacterbymultiplexrealtimepcrinstoolculturesamplesinouagadougouburkinafaso |