Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application
Limbal stem cells (LSC) are the progenitor cells that maintain the transparency of the cornea. Limbal stem cell deficiency (LSCD) leads to corneal opacity, inflammation, scarring, and blindness. A clinical approach to treat this condition consists in LSC transplantation (LSCT) after ex vivo expansio...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/3032128 |
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| author | Nuria Nieto-Nicolau Eva M. Martínez-Conesa Ricardo P. Casaroli-Marano |
| author_facet | Nuria Nieto-Nicolau Eva M. Martínez-Conesa Ricardo P. Casaroli-Marano |
| author_sort | Nuria Nieto-Nicolau |
| collection | DOAJ |
| description | Limbal stem cells (LSC) are the progenitor cells that maintain the transparency of the cornea. Limbal stem cell deficiency (LSCD) leads to corneal opacity, inflammation, scarring, and blindness. A clinical approach to treat this condition consists in LSC transplantation (LSCT) after ex vivo expansion of LSC. In unilateral LSCD, an autologous transplant is possible, but cases of bilateral LSCD require allogenic LSCT. Cadaveric donors represent the most important source of LSC allografts for treatment of bilateral LSCD when living relative donors are not available. To evaluate the suitability of aged cadaveric donors for LSCT, we compared three pools of LSC from donors of different ages (<60 years, 60–75 years, and >75 years). We evaluated graft quality in terms of percent of p63-positive (p63+) cells by immunofluorescence, colony forming efficiency, and mRNA and protein expression of p63, PAX6, Wnt7a, E-cadherin, and cytokeratin (CK) 12, CK3, and CK19. The results showed that LSC cultures from aged donors can express ≥3% of p63+ cells—considered as the minimum value for predicting favorable clinical outcomes after LSCT—suggesting that these cells could be a suitable source of LSC for transplantation. Our results also indicate the need to evaluate LSC graft quality criteria for each donor. |
| format | Article |
| id | doaj-art-f95e24f7eb20479d8b535af40724e775 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f95e24f7eb20479d8b535af40724e7752025-02-03T01:03:19ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/30321283032128Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical ApplicationNuria Nieto-Nicolau0Eva M. Martínez-Conesa1Ricardo P. Casaroli-Marano2CellTec-UB, Department of Cell Biology, University of Barcelona, Barcelona, SpainBarcelona Tissue Bank (BTB), Banc de Sang I Teixits (BST, GenCAT), Barcelona, SpainCellTec-UB, Department of Cell Biology, University of Barcelona, Barcelona, SpainLimbal stem cells (LSC) are the progenitor cells that maintain the transparency of the cornea. Limbal stem cell deficiency (LSCD) leads to corneal opacity, inflammation, scarring, and blindness. A clinical approach to treat this condition consists in LSC transplantation (LSCT) after ex vivo expansion of LSC. In unilateral LSCD, an autologous transplant is possible, but cases of bilateral LSCD require allogenic LSCT. Cadaveric donors represent the most important source of LSC allografts for treatment of bilateral LSCD when living relative donors are not available. To evaluate the suitability of aged cadaveric donors for LSCT, we compared three pools of LSC from donors of different ages (<60 years, 60–75 years, and >75 years). We evaluated graft quality in terms of percent of p63-positive (p63+) cells by immunofluorescence, colony forming efficiency, and mRNA and protein expression of p63, PAX6, Wnt7a, E-cadherin, and cytokeratin (CK) 12, CK3, and CK19. The results showed that LSC cultures from aged donors can express ≥3% of p63+ cells—considered as the minimum value for predicting favorable clinical outcomes after LSCT—suggesting that these cells could be a suitable source of LSC for transplantation. Our results also indicate the need to evaluate LSC graft quality criteria for each donor.http://dx.doi.org/10.1155/2016/3032128 |
| spellingShingle | Nuria Nieto-Nicolau Eva M. Martínez-Conesa Ricardo P. Casaroli-Marano Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application Stem Cells International |
| title | Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application |
| title_full | Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application |
| title_fullStr | Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application |
| title_full_unstemmed | Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application |
| title_short | Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application |
| title_sort | limbal stem cells from aged donors are a suitable source for clinical application |
| url | http://dx.doi.org/10.1155/2016/3032128 |
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