Marker-assisted identification of maize genotypes with improved protein quality
Currently, more than 70 % of maize is used for food and fodder; therefore, grain quality improvement can increase its nutritive and energy value. Deficiency of two essential amino acids (lysine and tryptophan) significantly reduces the nutritional quality of maize proteins. However, in comparison to...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-07-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/416 |
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| author | O. A. Orlovskaya S. V. Kubrak S. I. Vakula L. V. Khotyleva A. V. Kilchevsky |
| author_facet | O. A. Orlovskaya S. V. Kubrak S. I. Vakula L. V. Khotyleva A. V. Kilchevsky |
| author_sort | O. A. Orlovskaya |
| collection | DOAJ |
| description | Currently, more than 70 % of maize is used for food and fodder; therefore, grain quality improvement can increase its nutritive and energy value. Deficiency of two essential amino acids (lysine and tryptophan) significantly reduces the nutritional quality of maize proteins. However, in comparison to conventional maize varieties, opaque2 (o2) mutants have greater contents of lysine and tryptophan in their endosperm proteins and their bioavailability is better. The aim of the study was identification of maize accessions with high-quality protein. A collection of maize accessions of various ecogeographical origins was studied by molecular methods. This approach was expected to improve maize breeding efficiency. We collected 54 maize genotypes differing in grain quality performance. Amplification with three specific markers to the opaque-2 gene (phi057, phi112 and umc1066) revealed homozygous recessive o2 genotypes, associated with improved nutritional quality of the protein. UREA-PAG electrophoresis of zein proteins was used for Quality Protein Maize (QPM) identification. In addition to the mutant o2 allele, QPM contains genetic modifiers that convert starchy endosperm of o2 mutant to the hard vitreous phenotype. The selected QPM accessions are of interest for maize breeding programs aimed at grain quality improvement. The use of the markers to o2 and modifier genes accelerates the development of QPM varieties and significantly reduces the labor and financial costs of their production. |
| format | Article |
| id | doaj-art-f91318e3d1394e4b9359fa3edb30426b |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-f91318e3d1394e4b9359fa3edb30426b2025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-07-0119333333810.18699/VJ15.043375Marker-assisted identification of maize genotypes with improved protein qualityO. A. Orlovskaya0S. V. Kubrak1S. I. Vakula2L. V. Khotyleva3A. V. Kilchevsky4Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Republic of BelarusInstitute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Republic of BelarusInstitute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Republic of BelarusInstitute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Republic of BelarusInstitute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Republic of BelarusCurrently, more than 70 % of maize is used for food and fodder; therefore, grain quality improvement can increase its nutritive and energy value. Deficiency of two essential amino acids (lysine and tryptophan) significantly reduces the nutritional quality of maize proteins. However, in comparison to conventional maize varieties, opaque2 (o2) mutants have greater contents of lysine and tryptophan in their endosperm proteins and their bioavailability is better. The aim of the study was identification of maize accessions with high-quality protein. A collection of maize accessions of various ecogeographical origins was studied by molecular methods. This approach was expected to improve maize breeding efficiency. We collected 54 maize genotypes differing in grain quality performance. Amplification with three specific markers to the opaque-2 gene (phi057, phi112 and umc1066) revealed homozygous recessive o2 genotypes, associated with improved nutritional quality of the protein. UREA-PAG electrophoresis of zein proteins was used for Quality Protein Maize (QPM) identification. In addition to the mutant o2 allele, QPM contains genetic modifiers that convert starchy endosperm of o2 mutant to the hard vitreous phenotype. The selected QPM accessions are of interest for maize breeding programs aimed at grain quality improvement. The use of the markers to o2 and modifier genes accelerates the development of QPM varieties and significantly reduces the labor and financial costs of their production.https://vavilov.elpub.ru/jour/article/view/416maizeqpm genotypessr markersureapagezeins. |
| spellingShingle | O. A. Orlovskaya S. V. Kubrak S. I. Vakula L. V. Khotyleva A. V. Kilchevsky Marker-assisted identification of maize genotypes with improved protein quality Вавиловский журнал генетики и селекции maize qpm genotype ssr markers ureapage zeins. |
| title | Marker-assisted identification of maize genotypes with improved protein quality |
| title_full | Marker-assisted identification of maize genotypes with improved protein quality |
| title_fullStr | Marker-assisted identification of maize genotypes with improved protein quality |
| title_full_unstemmed | Marker-assisted identification of maize genotypes with improved protein quality |
| title_short | Marker-assisted identification of maize genotypes with improved protein quality |
| title_sort | marker assisted identification of maize genotypes with improved protein quality |
| topic | maize qpm genotype ssr markers ureapage zeins. |
| url | https://vavilov.elpub.ru/jour/article/view/416 |
| work_keys_str_mv | AT oaorlovskaya markerassistedidentificationofmaizegenotypeswithimprovedproteinquality AT svkubrak markerassistedidentificationofmaizegenotypeswithimprovedproteinquality AT sivakula markerassistedidentificationofmaizegenotypeswithimprovedproteinquality AT lvkhotyleva markerassistedidentificationofmaizegenotypeswithimprovedproteinquality AT avkilchevsky markerassistedidentificationofmaizegenotypeswithimprovedproteinquality |