loxP-Directed Cloning: Use of Cre Recombinase as a Universal Restriction Enzyme
We have developed a novel way to use the Cre/loxP system for in vitro manipulation of DNA and a technique to clone DNA into circular episomes. The method is fast, reliable, and allows flexible cloning of DNA fragments into episomes containing a loxP site. We show that a loxP site can serve as a univ...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01314rr02 |
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