Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from <i>Geum urbanum</i> L. on the Phenotype Inhibition of the <i>Pseudomonas aeruginosa</i> Las/RhI Quorum Sensing System
<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that causes nosocomial infections of the urinary tract, upper respiratory tract, gastrointestinal tract, central nervous system, etc. It is possible to develop bacteremia and sepsis in immunocompromised patients. A major problem...
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2025-01-01
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| author | Lyudmila Dimitrova Milka Mileva Almira Georgieva Elina Tzvetanova Milena Popova Vassya Bankova Hristo Najdenski |
| author_facet | Lyudmila Dimitrova Milka Mileva Almira Georgieva Elina Tzvetanova Milena Popova Vassya Bankova Hristo Najdenski |
| author_sort | Lyudmila Dimitrova |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that causes nosocomial infections of the urinary tract, upper respiratory tract, gastrointestinal tract, central nervous system, etc. It is possible to develop bacteremia and sepsis in immunocompromised patients. A major problem in treatment is the development of antibiotic resistance. Therefore, new preparations of natural origin are sought, such as plant extracts, which are phytocomplexes and to which it is practically impossible to develop resistance. <i>Geum urbanum</i> L. (<i>Rosacea</i>) is a perennial herb known for many biological properties. This study aimed to investigate the redox-modulating capacity and effect of ethyl acetate (EtOAc) extracts from roots (EtOAcR) and aerial parts (EtOAcAP) of the Bulgarian plant on the phenotype inhibition of the <i>P. aeruginosa</i> Las/RhI quorum sensing (QS) system, which primarily determines drug resistance in pathogenic bacteria, including biofilm formation, motility, and pigment production. We performed QS assays to account for the effects of the two EtOAc extracts. At sub-minimal inhibitory concentrations (sub-MICs) ranging from 1.56 to 6.25 mg/mL, the biofilm formation was inhibited 85% and 84% by EtOAcR and 62% and 39% by EtOAcAP extracts, respectively. At the same sub-MICs, the pyocyanin synthesis was inhibited by 17–27% after treatment with EtOAcAP and 26–30% with EtOAcR extracts. The motility was fully inhibited at 3.12 mg/mL and 6.25 mg/mL (sub-MICs). We investigated the inhibitory potential of <i>las</i>I, <i>las</i>R, <i>rhi</i>I, and <i>rhi</i>R gene expression in biofilm and pyocyanin probes with the PCR method. Interestingly, the genes were inhibited by two extracts at 3.12 mg/mL and 6.25 mg/mL. Antiradical studies, assessed by DPPH, CUPRAC, and ABTS radical scavenging methods and superoxide anion inhibition showed that EtOAcAP extract has effective antioxidant capacity. These results could help in the development of new phytocomplexes that could be applied as biocontrol agents to inhibit the phenotype of the <i>P. aeruginosa</i> QS system and other antibiotic-resistant pathogens. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-f818bb3494904e62b6dc5261321888c82025-01-24T13:46:47ZengMDPI AGPlants2223-77472025-01-0114221310.3390/plants14020213Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from <i>Geum urbanum</i> L. on the Phenotype Inhibition of the <i>Pseudomonas aeruginosa</i> Las/RhI Quorum Sensing SystemLyudmila Dimitrova0Milka Mileva1Almira Georgieva2Elina Tzvetanova3Milena Popova4Vassya Bankova5Hristo Najdenski6The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria<i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that causes nosocomial infections of the urinary tract, upper respiratory tract, gastrointestinal tract, central nervous system, etc. It is possible to develop bacteremia and sepsis in immunocompromised patients. A major problem in treatment is the development of antibiotic resistance. Therefore, new preparations of natural origin are sought, such as plant extracts, which are phytocomplexes and to which it is practically impossible to develop resistance. <i>Geum urbanum</i> L. (<i>Rosacea</i>) is a perennial herb known for many biological properties. This study aimed to investigate the redox-modulating capacity and effect of ethyl acetate (EtOAc) extracts from roots (EtOAcR) and aerial parts (EtOAcAP) of the Bulgarian plant on the phenotype inhibition of the <i>P. aeruginosa</i> Las/RhI quorum sensing (QS) system, which primarily determines drug resistance in pathogenic bacteria, including biofilm formation, motility, and pigment production. We performed QS assays to account for the effects of the two EtOAc extracts. At sub-minimal inhibitory concentrations (sub-MICs) ranging from 1.56 to 6.25 mg/mL, the biofilm formation was inhibited 85% and 84% by EtOAcR and 62% and 39% by EtOAcAP extracts, respectively. At the same sub-MICs, the pyocyanin synthesis was inhibited by 17–27% after treatment with EtOAcAP and 26–30% with EtOAcR extracts. The motility was fully inhibited at 3.12 mg/mL and 6.25 mg/mL (sub-MICs). We investigated the inhibitory potential of <i>las</i>I, <i>las</i>R, <i>rhi</i>I, and <i>rhi</i>R gene expression in biofilm and pyocyanin probes with the PCR method. Interestingly, the genes were inhibited by two extracts at 3.12 mg/mL and 6.25 mg/mL. Antiradical studies, assessed by DPPH, CUPRAC, and ABTS radical scavenging methods and superoxide anion inhibition showed that EtOAcAP extract has effective antioxidant capacity. These results could help in the development of new phytocomplexes that could be applied as biocontrol agents to inhibit the phenotype of the <i>P. aeruginosa</i> QS system and other antibiotic-resistant pathogens.https://www.mdpi.com/2223-7747/14/2/213<i>Geum urbanum</i> L.ethyl acetate roots and aerial pats extracts<i>Pseudomonas aeruginosa</i>biofilmspyocyanin productionredox-modulating capacity |
| spellingShingle | Lyudmila Dimitrova Milka Mileva Almira Georgieva Elina Tzvetanova Milena Popova Vassya Bankova Hristo Najdenski Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from <i>Geum urbanum</i> L. on the Phenotype Inhibition of the <i>Pseudomonas aeruginosa</i> Las/RhI Quorum Sensing System Plants <i>Geum urbanum</i> L. ethyl acetate roots and aerial pats extracts <i>Pseudomonas aeruginosa</i> biofilms pyocyanin production redox-modulating capacity |
| title | Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from <i>Geum urbanum</i> L. on the Phenotype Inhibition of the <i>Pseudomonas aeruginosa</i> Las/RhI Quorum Sensing System |
| title_full | Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from <i>Geum urbanum</i> L. on the Phenotype Inhibition of the <i>Pseudomonas aeruginosa</i> Las/RhI Quorum Sensing System |
| title_fullStr | Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from <i>Geum urbanum</i> L. on the Phenotype Inhibition of the <i>Pseudomonas aeruginosa</i> Las/RhI Quorum Sensing System |
| title_full_unstemmed | Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from <i>Geum urbanum</i> L. on the Phenotype Inhibition of the <i>Pseudomonas aeruginosa</i> Las/RhI Quorum Sensing System |
| title_short | Redox-Modulating Capacity and Effect of Ethyl Acetate Roots and Aerial Parts Extracts from <i>Geum urbanum</i> L. on the Phenotype Inhibition of the <i>Pseudomonas aeruginosa</i> Las/RhI Quorum Sensing System |
| title_sort | redox modulating capacity and effect of ethyl acetate roots and aerial parts extracts from i geum urbanum i l on the phenotype inhibition of the i pseudomonas aeruginosa i las rhi quorum sensing system |
| topic | <i>Geum urbanum</i> L. ethyl acetate roots and aerial pats extracts <i>Pseudomonas aeruginosa</i> biofilms pyocyanin production redox-modulating capacity |
| url | https://www.mdpi.com/2223-7747/14/2/213 |
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