Influence of high-load shocks on achieving mainstream partial nitrification: Microbial community succession
Driving microbial community succession through the regulation of operational strategies is crucial for achieving partial nitrification (PN) in municipal wastewater. However, at present, there is a decoupling between the strategic regulation of PN systems and the succession characteristics of the mic...
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Elsevier
2025-05-01
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| Series: | Water Research X |
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| author | Chenjie Jia Jialin Li Zhaoyang Li Liang Zhang |
| author_facet | Chenjie Jia Jialin Li Zhaoyang Li Liang Zhang |
| author_sort | Chenjie Jia |
| collection | DOAJ |
| description | Driving microbial community succession through the regulation of operational strategies is crucial for achieving partial nitrification (PN) in municipal wastewater. However, at present, there is a decoupling between the strategic regulation of PN systems and the succession characteristics of the microbial community. This study examined the correlation between microbial community succession and PN performance under two high-load shocks (HLS1 and HLS2) treating actual sewage. During HLS1, the influent organic loading rate (OLR) and nitrogen loading rate (NLR) increased from 116.7 ± 37.7 to 219.7 ± 24.7 mg COD/(g VSS·d) and 0.21±0.02 to 0.33±0.02 kg N/m3/d respectively, with the nitrite concentration and nitrite accumulation ratio only reaching 11.7 ± 2.7 mg/L and 49.3 ± 13.9 %, respectively. During HLS2, the influent OLR and NLR increased from 123.5 ± 17.2 to 300.3 ± 49.2 mg COD/(g VSS·d) and 0.19±0.03 to 0.32±0.03 kg N/m3/d respectively, resulting in a nitrite accumulation ratio of 89.4 ± 10.7 %. The system achieved efficient PN performance and sustained for 124 days. High-throughput sequencing results showed that community diversity remained consistently high, and the community composition returned to its initial state following a minor succession during HLS1. During HLS2, the high-load shock reduced the richness and evenness of the microbial community. The community underwent succession in a new direction, leading to community composition and function changes. The results indicate that the realization, stabilization, and disruption of PN are influenced not only by operational parameters but also by microbial community structure. |
| format | Article |
| id | doaj-art-f7fc73ca682d431a828c777989126bae |
| institution | Kabale University |
| issn | 2589-9147 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
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| series | Water Research X |
| spelling | doaj-art-f7fc73ca682d431a828c777989126bae2025-01-23T05:27:37ZengElsevierWater Research X2589-91472025-05-0127100304Influence of high-load shocks on achieving mainstream partial nitrification: Microbial community successionChenjie Jia0Jialin Li1Zhaoyang Li2Liang Zhang3National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, PR ChinaCorresponding authors. Beijing University of Technology, Beijing 100124, PR China; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, PR ChinaNational Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, PR ChinaCorresponding authors. Beijing University of Technology, Beijing 100124, PR China; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, PR ChinaDriving microbial community succession through the regulation of operational strategies is crucial for achieving partial nitrification (PN) in municipal wastewater. However, at present, there is a decoupling between the strategic regulation of PN systems and the succession characteristics of the microbial community. This study examined the correlation between microbial community succession and PN performance under two high-load shocks (HLS1 and HLS2) treating actual sewage. During HLS1, the influent organic loading rate (OLR) and nitrogen loading rate (NLR) increased from 116.7 ± 37.7 to 219.7 ± 24.7 mg COD/(g VSS·d) and 0.21±0.02 to 0.33±0.02 kg N/m3/d respectively, with the nitrite concentration and nitrite accumulation ratio only reaching 11.7 ± 2.7 mg/L and 49.3 ± 13.9 %, respectively. During HLS2, the influent OLR and NLR increased from 123.5 ± 17.2 to 300.3 ± 49.2 mg COD/(g VSS·d) and 0.19±0.03 to 0.32±0.03 kg N/m3/d respectively, resulting in a nitrite accumulation ratio of 89.4 ± 10.7 %. The system achieved efficient PN performance and sustained for 124 days. High-throughput sequencing results showed that community diversity remained consistently high, and the community composition returned to its initial state following a minor succession during HLS1. During HLS2, the high-load shock reduced the richness and evenness of the microbial community. The community underwent succession in a new direction, leading to community composition and function changes. The results indicate that the realization, stabilization, and disruption of PN are influenced not only by operational parameters but also by microbial community structure.http://www.sciencedirect.com/science/article/pii/S2589914725000040Stable nitritationMicrobial ecologyOrganic loading rateNitrogen loading rateMunicipal sewage |
| spellingShingle | Chenjie Jia Jialin Li Zhaoyang Li Liang Zhang Influence of high-load shocks on achieving mainstream partial nitrification: Microbial community succession Water Research X Stable nitritation Microbial ecology Organic loading rate Nitrogen loading rate Municipal sewage |
| title | Influence of high-load shocks on achieving mainstream partial nitrification: Microbial community succession |
| title_full | Influence of high-load shocks on achieving mainstream partial nitrification: Microbial community succession |
| title_fullStr | Influence of high-load shocks on achieving mainstream partial nitrification: Microbial community succession |
| title_full_unstemmed | Influence of high-load shocks on achieving mainstream partial nitrification: Microbial community succession |
| title_short | Influence of high-load shocks on achieving mainstream partial nitrification: Microbial community succession |
| title_sort | influence of high load shocks on achieving mainstream partial nitrification microbial community succession |
| topic | Stable nitritation Microbial ecology Organic loading rate Nitrogen loading rate Municipal sewage |
| url | http://www.sciencedirect.com/science/article/pii/S2589914725000040 |
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