TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological Cells
Tumour necrosis factor-α, interferon-γ and interleukin-4 are critical cytokines in regulating the immune responses against infections and tumours. In this study, we investigated the effects of three cytokines on CD40 expression in Myb-transformed hematological cells and their regulatory roles in pro...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/621969 |
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| author | Wenyi Gu Jiezhong Chen Lei Yang Kong-Nan Zhao |
| author_facet | Wenyi Gu Jiezhong Chen Lei Yang Kong-Nan Zhao |
| author_sort | Wenyi Gu |
| collection | DOAJ |
| description | Tumour necrosis factor-α, interferon-γ and interleukin-4 are critical cytokines in regulating the immune responses against infections and tumours. In this study, we investigated the effects of three cytokines on CD40 expression in Myb-transformed hematological cells and their regulatory roles in promoting these cells into dendritic cells. We observed that both interleukin-4 and interferon-γ increased CD40 expression in these hematological cells in a dose-dependent manner, although the concentration required for interleukin-4 was significantly higher than that for interferon-γ. We found that tumour necrosis factor-α promoted CD40 expression induced by interferon-γ, but not by interleukin-4. Our data showed that tumour necrosis factor-α plus interferon-γ-treated Myb-transformed hematological cells had the greatest ability to take up and process the model antigen DQ-Ovalbumin. Tumour necrosis factor-α also increased the ability of interferon-γ to produce the mixed lymphocyte reaction to allogenic T cells. Furthermore, only cotreatment with tumour necrosis factor-α and interferon-γ induced Myb-transformed hematological cells to express interleukin-6. These results suggest that tumour necrosis factor-α plays a key regulatory role in the development of dendritic cells from hematological progenitor cells induced by interferon-γ. |
| format | Article |
| id | doaj-art-f775cab51fb142c4ad7bb1f507b4b3e2 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f775cab51fb142c4ad7bb1f507b4b3e22025-02-03T06:10:53ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/621969621969TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological CellsWenyi Gu0Jiezhong Chen1Lei Yang2Kong-Nan Zhao3Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, AustraliaIllawarra Health and Medical Research Institute, University of Wollongong, NSW 2522, AustraliaSchool of Medicine and Medical Management, Hangzhou Normal University, Hangzhou 310036, ChinaUQ Centre for Clinical Research, The University of Queensland, Herston, Brisbane, QLD 4029, AustraliaTumour necrosis factor-α, interferon-γ and interleukin-4 are critical cytokines in regulating the immune responses against infections and tumours. In this study, we investigated the effects of three cytokines on CD40 expression in Myb-transformed hematological cells and their regulatory roles in promoting these cells into dendritic cells. We observed that both interleukin-4 and interferon-γ increased CD40 expression in these hematological cells in a dose-dependent manner, although the concentration required for interleukin-4 was significantly higher than that for interferon-γ. We found that tumour necrosis factor-α promoted CD40 expression induced by interferon-γ, but not by interleukin-4. Our data showed that tumour necrosis factor-α plus interferon-γ-treated Myb-transformed hematological cells had the greatest ability to take up and process the model antigen DQ-Ovalbumin. Tumour necrosis factor-α also increased the ability of interferon-γ to produce the mixed lymphocyte reaction to allogenic T cells. Furthermore, only cotreatment with tumour necrosis factor-α and interferon-γ induced Myb-transformed hematological cells to express interleukin-6. These results suggest that tumour necrosis factor-α plays a key regulatory role in the development of dendritic cells from hematological progenitor cells induced by interferon-γ.http://dx.doi.org/10.1100/2012/621969 |
| spellingShingle | Wenyi Gu Jiezhong Chen Lei Yang Kong-Nan Zhao TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological Cells The Scientific World Journal |
| title | TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological Cells |
| title_full | TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological Cells |
| title_fullStr | TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological Cells |
| title_full_unstemmed | TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological Cells |
| title_short | TNF-α Promotes IFN-γ-Induced CD40 Expression and Antigen Process in Myb-Transformed Hematological Cells |
| title_sort | tnf α promotes ifn γ induced cd40 expression and antigen process in myb transformed hematological cells |
| url | http://dx.doi.org/10.1100/2012/621969 |
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