A quick in vitro pathway from prokaryotic genomic libraries to enzyme discovery
Screening of prokaryotic genomes in order to identify enzymes with a desired catalytic activity can be performed in vivo in bacterial cells. We propose a strategy of in vitro expression screening of large prokaryotic genomic libraries based on Escherichia coli cell-free transcription-translation sys...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2008-07-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112820 |
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