New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties
Plant-derived nanovesicles represent a novel approach in the field of plant-derived biomaterials, offering a sustainable and biocompatible option for various biomedical applications. The unique properties of these vesicles, such as their ability to encapsulate bioactive compounds, make them suitable...
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2024-10-01
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| author | Flores Naselli Sara Volpes Paola Sofia Cardinale Fabio Salvatore Palumbo Francesco Cancilla Francesco Lopresti Valeria Villanova Antonella Girgenti Domenico Nuzzo Fabio Caradonna Pasquale Picone |
| author_facet | Flores Naselli Sara Volpes Paola Sofia Cardinale Fabio Salvatore Palumbo Francesco Cancilla Francesco Lopresti Valeria Villanova Antonella Girgenti Domenico Nuzzo Fabio Caradonna Pasquale Picone |
| author_sort | Flores Naselli |
| collection | DOAJ |
| description | Plant-derived nanovesicles represent a novel approach in the field of plant-derived biomaterials, offering a sustainable and biocompatible option for various biomedical applications. The unique properties of these vesicles, such as their ability to encapsulate bioactive compounds, make them suitable for therapeutic, cosmetic, and nutraceutical purposes. In this study, we have, for the first time, successfully bio-fabricated vesicles derived from Opuntia ficus-indica (FicoVes) using an efficient and cost-effective method. Characterized by a size of approximately of 114 nm and a negative zeta potential of −20.9 mV, FicoVes exhibited excellent biocompatibility and hemocompatibility, showing no reduction in the viability of human and animal cells. Our results showed that FicoVes possess significant antioxidant properties as they reduced ROS generation in TBH-stimulated cells. FicoVes displayed anti-inflammatory properties by reducing the expression of pro-inflammatory cytokines (Il 1β, TNF α) and enhancing the expression of anti-inflammatory cytokines (IL4, IL10) following an inflammatory stimulus. Furthermore, FicoVes accelerated epithelial wound closure in L929 fibroblast monolayers in a dose-dependent manner, highlighting their potential role in tissue repair. This study establishes FicoVes as a promising candidate for nutrigenomic applications, particularly in the context of inflammation-related disorders and wound healing. Further research, including in vivo studies, is essential to validate these findings and fully explore their therapeutic potential. |
| format | Article |
| id | doaj-art-f66af4ce277541c48d41324da44f451f |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-f66af4ce277541c48d41324da44f451f2024-11-08T14:34:24ZengMDPI AGCells2073-44092024-10-011321175610.3390/cells13211756New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic PropertiesFlores Naselli0Sara Volpes1Paola Sofia Cardinale2Fabio Salvatore Palumbo3Francesco Cancilla4Francesco Lopresti5Valeria Villanova6Antonella Girgenti7Domenico Nuzzo8Fabio Caradonna9Pasquale Picone10Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze Building 16, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze Building 16, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze Building 16, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze Building 16, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze Building 16, 90128 Palermo, ItalyDepartment of Engineering, University of Palermo, Viale delle Scienze, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze Building 16, 90128 Palermo, ItalyInstitute for Biomedical Research and Innovation, National Research Council of Italy, Via U. La Malfa, 153, 90146 Palermo, ItalyInstitute for Biomedical Research and Innovation, National Research Council of Italy, Via U. La Malfa, 153, 90146 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze Building 16, 90128 Palermo, ItalyInstitute for Biomedical Research and Innovation, National Research Council of Italy, Via U. La Malfa, 153, 90146 Palermo, ItalyPlant-derived nanovesicles represent a novel approach in the field of plant-derived biomaterials, offering a sustainable and biocompatible option for various biomedical applications. The unique properties of these vesicles, such as their ability to encapsulate bioactive compounds, make them suitable for therapeutic, cosmetic, and nutraceutical purposes. In this study, we have, for the first time, successfully bio-fabricated vesicles derived from Opuntia ficus-indica (FicoVes) using an efficient and cost-effective method. Characterized by a size of approximately of 114 nm and a negative zeta potential of −20.9 mV, FicoVes exhibited excellent biocompatibility and hemocompatibility, showing no reduction in the viability of human and animal cells. Our results showed that FicoVes possess significant antioxidant properties as they reduced ROS generation in TBH-stimulated cells. FicoVes displayed anti-inflammatory properties by reducing the expression of pro-inflammatory cytokines (Il 1β, TNF α) and enhancing the expression of anti-inflammatory cytokines (IL4, IL10) following an inflammatory stimulus. Furthermore, FicoVes accelerated epithelial wound closure in L929 fibroblast monolayers in a dose-dependent manner, highlighting their potential role in tissue repair. This study establishes FicoVes as a promising candidate for nutrigenomic applications, particularly in the context of inflammation-related disorders and wound healing. Further research, including in vivo studies, is essential to validate these findings and fully explore their therapeutic potential.https://www.mdpi.com/2073-4409/13/21/1756plant-derived nanovesicles (PDNVs)bioactive phytocompoundsantioxidant and anti-inflammatory propertiesnutrigenomicsepithelial repair |
| spellingShingle | Flores Naselli Sara Volpes Paola Sofia Cardinale Fabio Salvatore Palumbo Francesco Cancilla Francesco Lopresti Valeria Villanova Antonella Girgenti Domenico Nuzzo Fabio Caradonna Pasquale Picone New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties Cells plant-derived nanovesicles (PDNVs) bioactive phytocompounds antioxidant and anti-inflammatory properties nutrigenomics epithelial repair |
| title | New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties |
| title_full | New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties |
| title_fullStr | New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties |
| title_full_unstemmed | New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties |
| title_short | New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties |
| title_sort | new nanovesicles from prickly pear fruit juice a resource with antioxidant anti inflammatory and nutrigenomic properties |
| topic | plant-derived nanovesicles (PDNVs) bioactive phytocompounds antioxidant and anti-inflammatory properties nutrigenomics epithelial repair |
| url | https://www.mdpi.com/2073-4409/13/21/1756 |
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