Mapping Gene Expression in Whole Larval Brains of <i>Bicyclus anynana</i> Butterflies

Butterfly larvae display intricate cognitive capacities and behaviors, but relatively little is known about how those behaviors alter their brains at the molecular level. Here, we optimized a hybridization chain reaction 3.0 (HCR v3.0) protocol to visualize the expression of multiple RNA molecules i...

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Bibliographic Details
Main Authors: Tirtha Das Banerjee, Linwan Zhang, Antónia Monteiro
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Methods and Protocols
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Online Access:https://www.mdpi.com/2409-9279/8/2/31
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