PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants
This study aimed to assess the prevalence of Babesia ovis infection in adult Rhipicephalus bursa and small ruminants in West Azerbaijan province, Iran. Blood samples were collected from 280 sheep and 122 goats of forty randomly selected flocks. Specific B. ovis fragment was detected in 67 animals (1...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/294704 |
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| author | Bijan Esmaeilnejad Mousa Tavassoli Siamak Asri-Rezaei Bahram Dalir-Naghadeh Karim Mardani Ghader Jalilzadeh-Amin Mostafa Golabi Jafar Arjmand |
| author_facet | Bijan Esmaeilnejad Mousa Tavassoli Siamak Asri-Rezaei Bahram Dalir-Naghadeh Karim Mardani Ghader Jalilzadeh-Amin Mostafa Golabi Jafar Arjmand |
| author_sort | Bijan Esmaeilnejad |
| collection | DOAJ |
| description | This study aimed to assess the prevalence of Babesia ovis infection in adult Rhipicephalus bursa and small ruminants in West Azerbaijan province, Iran. Blood samples were collected from 280 sheep and 122 goats of forty randomly selected flocks. Specific B. ovis fragment was detected in 67 animals (16.7%), of which 52 animals (18.6%) were sheep and 15 animals (12.2%) goats (P<0.05). Of the 848 R. bursa collected from naturally infested small ruminants and farm dogs, Babesia ovis was detected by PCR in salivary glands of 94 adult ticks. The frequency of B. ovis infection was higher in flocks with tick in comparison with animals without tick (P<0.05). Positive amplification from blood of ruminants, ticks, oviposition ticks, eggs, and larvae was subjected to restriction digestion with HphI. One RFLP profile was produced. The PCR-RFLP results indicated that one strain of B. ovis exists in this area. The results showed that the PCR was useful method to investigate the epidemiology of small ruminants’ babesiosis. Furthermore, R. Bursa, which can transovarially transmit B. ovis and as well as being widely distributed in West Azerbaijan province, Iran, might play an important role in the field as a natural vector of B. ovis. |
| format | Article |
| id | doaj-art-f52a39eccdc34bf8af413ac225c2eed8 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-f52a39eccdc34bf8af413ac225c2eed82025-02-03T05:59:36ZengWileyJournal of Parasitology Research2090-00232090-00312014-01-01201410.1155/2014/294704294704PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small RuminantsBijan Esmaeilnejad0Mousa Tavassoli1Siamak Asri-Rezaei2Bahram Dalir-Naghadeh3Karim Mardani4Ghader Jalilzadeh-Amin5Mostafa Golabi6Jafar Arjmand7Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartments of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartments of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartments of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranThis study aimed to assess the prevalence of Babesia ovis infection in adult Rhipicephalus bursa and small ruminants in West Azerbaijan province, Iran. Blood samples were collected from 280 sheep and 122 goats of forty randomly selected flocks. Specific B. ovis fragment was detected in 67 animals (16.7%), of which 52 animals (18.6%) were sheep and 15 animals (12.2%) goats (P<0.05). Of the 848 R. bursa collected from naturally infested small ruminants and farm dogs, Babesia ovis was detected by PCR in salivary glands of 94 adult ticks. The frequency of B. ovis infection was higher in flocks with tick in comparison with animals without tick (P<0.05). Positive amplification from blood of ruminants, ticks, oviposition ticks, eggs, and larvae was subjected to restriction digestion with HphI. One RFLP profile was produced. The PCR-RFLP results indicated that one strain of B. ovis exists in this area. The results showed that the PCR was useful method to investigate the epidemiology of small ruminants’ babesiosis. Furthermore, R. Bursa, which can transovarially transmit B. ovis and as well as being widely distributed in West Azerbaijan province, Iran, might play an important role in the field as a natural vector of B. ovis.http://dx.doi.org/10.1155/2014/294704 |
| spellingShingle | Bijan Esmaeilnejad Mousa Tavassoli Siamak Asri-Rezaei Bahram Dalir-Naghadeh Karim Mardani Ghader Jalilzadeh-Amin Mostafa Golabi Jafar Arjmand PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants Journal of Parasitology Research |
| title | PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants |
| title_full | PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants |
| title_fullStr | PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants |
| title_full_unstemmed | PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants |
| title_short | PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants |
| title_sort | pcr based detection of babesia ovis in rhipicephalus bursa and small ruminants |
| url | http://dx.doi.org/10.1155/2014/294704 |
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