Mapping Surface Potential in DNA Aptamer–Neurochemical and Membrane–Ion Interactions on the SOS Substrate Using Terahertz Microscopy
In this study, we utilized a terahertz chemical microscope (TCM) to map surface potential changes induced by molecular interactions on silicon-on-sapphire (SOS) substrates. By functionalizing the SOS substrate with DNA aptamers and an ion-selective membrane, we successfully detected and visualized a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/46 |
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| author | Kosei Morita Yuta Mitsuda Sota Yoshida Toshihiko Kiwa Jin Wang |
| author_facet | Kosei Morita Yuta Mitsuda Sota Yoshida Toshihiko Kiwa Jin Wang |
| author_sort | Kosei Morita |
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| description | In this study, we utilized a terahertz chemical microscope (TCM) to map surface potential changes induced by molecular interactions on silicon-on-sapphire (SOS) substrates. By functionalizing the SOS substrate with DNA aptamers and an ion-selective membrane, we successfully detected and visualized aptamer–neurochemical complexes through the terahertz amplitude. Additionally, comparative studies of DNA aptamers in PBS buffer and artificial cerebrospinal fluid (aCSF) were performed by computational structure modeling and terahertz measurements. Beyond neurochemicals, we also investigated calcium ions, measuring their concentrations in PDMS-fabricated micro-wells using minimal sample volumes. Our results highlight the capability of TCM as a powerful, label-free, and sensitive platform for the probing and mapping of surface potential arising from molecular interactions, with broad implications for biomedical diagnostics and research. |
| format | Article |
| id | doaj-art-f4ac90521ebd424190a9f87782f87156 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-f4ac90521ebd424190a9f87782f871562025-01-24T13:25:32ZengMDPI AGBiosensors2079-63742025-01-011514610.3390/bios15010046Mapping Surface Potential in DNA Aptamer–Neurochemical and Membrane–Ion Interactions on the SOS Substrate Using Terahertz MicroscopyKosei Morita0Yuta Mitsuda1Sota Yoshida2Toshihiko Kiwa3Jin Wang4Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, JapanGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, JapanGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, JapanGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, JapanGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, JapanIn this study, we utilized a terahertz chemical microscope (TCM) to map surface potential changes induced by molecular interactions on silicon-on-sapphire (SOS) substrates. By functionalizing the SOS substrate with DNA aptamers and an ion-selective membrane, we successfully detected and visualized aptamer–neurochemical complexes through the terahertz amplitude. Additionally, comparative studies of DNA aptamers in PBS buffer and artificial cerebrospinal fluid (aCSF) were performed by computational structure modeling and terahertz measurements. Beyond neurochemicals, we also investigated calcium ions, measuring their concentrations in PDMS-fabricated micro-wells using minimal sample volumes. Our results highlight the capability of TCM as a powerful, label-free, and sensitive platform for the probing and mapping of surface potential arising from molecular interactions, with broad implications for biomedical diagnostics and research.https://www.mdpi.com/2079-6374/15/1/46terahertz chemical microscopesurface potentialDNA aptamer–neurochemical complexesmembrane–ion interactionsSOS substrateartificial cerebrospinal fluid |
| spellingShingle | Kosei Morita Yuta Mitsuda Sota Yoshida Toshihiko Kiwa Jin Wang Mapping Surface Potential in DNA Aptamer–Neurochemical and Membrane–Ion Interactions on the SOS Substrate Using Terahertz Microscopy Biosensors terahertz chemical microscope surface potential DNA aptamer–neurochemical complexes membrane–ion interactions SOS substrate artificial cerebrospinal fluid |
| title | Mapping Surface Potential in DNA Aptamer–Neurochemical and Membrane–Ion Interactions on the SOS Substrate Using Terahertz Microscopy |
| title_full | Mapping Surface Potential in DNA Aptamer–Neurochemical and Membrane–Ion Interactions on the SOS Substrate Using Terahertz Microscopy |
| title_fullStr | Mapping Surface Potential in DNA Aptamer–Neurochemical and Membrane–Ion Interactions on the SOS Substrate Using Terahertz Microscopy |
| title_full_unstemmed | Mapping Surface Potential in DNA Aptamer–Neurochemical and Membrane–Ion Interactions on the SOS Substrate Using Terahertz Microscopy |
| title_short | Mapping Surface Potential in DNA Aptamer–Neurochemical and Membrane–Ion Interactions on the SOS Substrate Using Terahertz Microscopy |
| title_sort | mapping surface potential in dna aptamer neurochemical and membrane ion interactions on the sos substrate using terahertz microscopy |
| topic | terahertz chemical microscope surface potential DNA aptamer–neurochemical complexes membrane–ion interactions SOS substrate artificial cerebrospinal fluid |
| url | https://www.mdpi.com/2079-6374/15/1/46 |
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