BLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay Targeting <i>pol</i> Region

BLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay Targeting <i>pol</i> Region

The proviral load (PVL) of the bovine leukemia virus (BLV) is a useful index for estimating disease progression and transmission risk. Real-time quantitative PCR techniques are widely used for PVL quantification. We previously developed a dual-target detection method, the “Liquid Dual-CoCoMo assay”,...

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Bibliographic Details
Main Authors: Sonoko Watanuki, Aronggaowa Bao, Etsuko Saitou, Kazuyuki Shoji, Masaki Izawa, Mitsuaki Okami, Yasunobu Matsumoto, Yoko Aida
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Pathogens
Subjects:
bovine leukemia virus (BLV)
proviral load (PVL)
quantitative real-time PCR (qPCR)
duplex
long terminal repeat (LTR)
<i>pol</i> gene
Online Access:https://www.mdpi.com/2076-0817/13/12/1111
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