Discrimination of SNPs in GC-rich regions using a modified hydrolysis probe chemistry protocol
Allelic discrimination using TaqMan 5′-nuclease assay chemistry has been in routine use for many years, and the catalog of Life Technologies— predesigned SNP genotyping assays now exceeds 4 million entries. However, predesigned assays are often not available for genomic regions with a high GC conten...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2014-12-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000114240 |
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