A chemical approach facilitates CRISPRa-only human iPSC generation and minimizes the number of targeted loci required

A chemical approach facilitates CRISPRa-only human iPSC generation and minimizes the number of targeted loci required

Aim: We explored the generation of human induced pluripotent stem cells (iPSCs) solely through the transcriptional activation of endogenous genes by CRISPR activation (CRISPRa). Methods: Minimal number of human-specific guide RNAs targeting a limited set of loci were used with a unique cocktail of s...

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Bibliographic Details
Main Authors: Ramzey Abujarour, Jason Dinella, Mochtar Pribadi, Lauren K Fong, Matthew Denholtz, Alma Gutierrez, Matt Haynes, Enaaya Mahmood, Tom T Lee, Sheng Ding, Bahram Valamehr
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Future Science OA
Subjects:
CRISPR
CRISPRa
human
induced pluripotent
reprogramming
small molecules
Online Access:https://www.tandfonline.com/doi/10.2144/fsoa-2023-0257
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https://www.tandfonline.com/doi/10.2144/fsoa-2023-0257

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