Synthesis, molecular docking, and anti-inflammatory evaluation of novel 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine derivatives as potent cysteinyl leukotriene receptor (CysLT2) antagonists

Cysteinyl leukotrienes (CysLTs), derived from arachidonic acid, play a crucial role in regulating inflammation. We synthesized a novel series of CysLT2 antagonists containing the benzo[d][1,3]dioxole moiety using a synthetic strategy based on the Mannich reaction, resulting in the formation of 4-(be...

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Main Authors: Areej M. Jaber, Mohammed M. Al-Mahadeen, Belal O. Al-Najjar, Raed A. Al-Qawasmeh
Format: Article
Language:English
Published: Pensoft Publishers 2025-02-01
Series:Pharmacia
Online Access:https://pharmacia.pensoft.net/article/143171/download/pdf/
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author Areej M. Jaber
Mohammed M. Al-Mahadeen
Belal O. Al-Najjar
Raed A. Al-Qawasmeh
author_facet Areej M. Jaber
Mohammed M. Al-Mahadeen
Belal O. Al-Najjar
Raed A. Al-Qawasmeh
author_sort Areej M. Jaber
collection DOAJ
description Cysteinyl leukotrienes (CysLTs), derived from arachidonic acid, play a crucial role in regulating inflammation. We synthesized a novel series of CysLT2 antagonists containing the benzo[d][1,3]dioxole moiety using a synthetic strategy based on the Mannich reaction, resulting in the formation of 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine. The structures of the synthesized compounds were characterized through NMR and HRMS analyses, and a plausible reaction mechanism was proposed. The inhibitory activities of all new compounds against CysLT2 were evaluated in vitro. Among them, compounds 4d and 4g exhibited the most potent inhibitory effects, with IC50 values of 18.7 μM and 15.5 μM, respectively. These findings were further supported by molecular docking studies, which highlighted the binding interactions of these compounds within the kinase’s active site. Graphical abstract:
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spelling doaj-art-f17a835b1bfc4375be1bd523314d06072025-08-20T02:17:09ZengPensoft PublishersPharmacia2603-557X2025-02-017211110.3897/pharmacia.72.e143171143171Synthesis, molecular docking, and anti-inflammatory evaluation of novel 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine derivatives as potent cysteinyl leukotriene receptor (CysLT2) antagonistsAreej M. Jaber0Mohammed M. Al-Mahadeen1Belal O. Al-Najjar2Raed A. Al-Qawasmeh3Al-Ahliyya Amman UniversityThe University of JordanAl-Ahliyya Amman UniversityUniversity of SharjahCysteinyl leukotrienes (CysLTs), derived from arachidonic acid, play a crucial role in regulating inflammation. We synthesized a novel series of CysLT2 antagonists containing the benzo[d][1,3]dioxole moiety using a synthetic strategy based on the Mannich reaction, resulting in the formation of 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine. The structures of the synthesized compounds were characterized through NMR and HRMS analyses, and a plausible reaction mechanism was proposed. The inhibitory activities of all new compounds against CysLT2 were evaluated in vitro. Among them, compounds 4d and 4g exhibited the most potent inhibitory effects, with IC50 values of 18.7 μM and 15.5 μM, respectively. These findings were further supported by molecular docking studies, which highlighted the binding interactions of these compounds within the kinase’s active site. Graphical abstract:https://pharmacia.pensoft.net/article/143171/download/pdf/
spellingShingle Areej M. Jaber
Mohammed M. Al-Mahadeen
Belal O. Al-Najjar
Raed A. Al-Qawasmeh
Synthesis, molecular docking, and anti-inflammatory evaluation of novel 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine derivatives as potent cysteinyl leukotriene receptor (CysLT2) antagonists
Pharmacia
title Synthesis, molecular docking, and anti-inflammatory evaluation of novel 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine derivatives as potent cysteinyl leukotriene receptor (CysLT2) antagonists
title_full Synthesis, molecular docking, and anti-inflammatory evaluation of novel 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine derivatives as potent cysteinyl leukotriene receptor (CysLT2) antagonists
title_fullStr Synthesis, molecular docking, and anti-inflammatory evaluation of novel 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine derivatives as potent cysteinyl leukotriene receptor (CysLT2) antagonists
title_full_unstemmed Synthesis, molecular docking, and anti-inflammatory evaluation of novel 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine derivatives as potent cysteinyl leukotriene receptor (CysLT2) antagonists
title_short Synthesis, molecular docking, and anti-inflammatory evaluation of novel 4-(benzo[d][1,3]dioxol-5-yloxy)-N,N-2-yn-1-amine derivatives as potent cysteinyl leukotriene receptor (CysLT2) antagonists
title_sort synthesis molecular docking and anti inflammatory evaluation of novel 4 benzo d 1 3 dioxol 5 yloxy n n 2 yn 1 amine derivatives as potent cysteinyl leukotriene receptor cyslt2 antagonists
url https://pharmacia.pensoft.net/article/143171/download/pdf/
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