miR-301a-mediated crosstalk between the Hedgehog and HIPPO/YAP signaling pathways promotes pancreatic cancer
Pancreatic ductal adenocarcinoma (PDAC) poses a significant challenge in oncology due to its dismal prognosis and limited therapeutic options. In this study, we investigated the role of miR-301a in facilitating crosstalk between the Hedgehog (Hh) and HIPPO/YAP signaling pathways during the progressi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Cancer Biology & Therapy |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15384047.2025.2457761 |
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| _version_ | 1832590506069065728 |
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| author | Bing Qi Yuqiong Wang Xian Zhu Yanfang Gong Jing Jin Hongyu Wu Xiaohua Man Feng Liu Wenzhu Yao Jun Gao |
| author_facet | Bing Qi Yuqiong Wang Xian Zhu Yanfang Gong Jing Jin Hongyu Wu Xiaohua Man Feng Liu Wenzhu Yao Jun Gao |
| author_sort | Bing Qi |
| collection | DOAJ |
| description | Pancreatic ductal adenocarcinoma (PDAC) poses a significant challenge in oncology due to its dismal prognosis and limited therapeutic options. In this study, we investigated the role of miR-301a in facilitating crosstalk between the Hedgehog (Hh) and HIPPO/YAP signaling pathways during the progression of PDAC. Our findings revealed that miR-301a served as a central regulatory node, targeting Gli1 within the Hh pathway and STK4 within the HIPPO/YAP pathway. Immunohistochemical and molecular analyses confirmed dysregulation of pathway components in pancreatic cancer, underscoring the pivotal role of miR-301a. Functional assays demonstrated the impact of miR-301a on cell proliferation and apoptosis, particularly in synergy with TNF-α. Overall, our study elucidated the intricate interplay between the Hh and HIPPO/YAP pathways mediated by miR-301a, providing valuable insights into potential therapeutic strategies for intervening in PDAC. |
| format | Article |
| id | doaj-art-f12f86746e094a8892024794085dcb3d |
| institution | Kabale University |
| issn | 1538-4047 1555-8576 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cancer Biology & Therapy |
| spelling | doaj-art-f12f86746e094a8892024794085dcb3d2025-01-23T17:58:08ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762025-12-0126110.1080/15384047.2025.2457761miR-301a-mediated crosstalk between the Hedgehog and HIPPO/YAP signaling pathways promotes pancreatic cancerBing Qi0Yuqiong Wang1Xian Zhu2Yanfang Gong3Jing Jin4Hongyu Wu5Xiaohua Man6Feng Liu7Wenzhu Yao8Jun Gao9Institute of Oncology, Second Affiliated Hospital, Xi’an Medical University, Xi’an, ChinaDepartment of Gastroenterology, The Hospital of 92608 People’s Liberation Army of China (PLA) Troops, Shanghai, ChinaDepartment of Gastroenterology, Ningbo Medical Center Lihuili Hospital, Ningbo, ChinaDepartment of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Gastroenterology, Tongchuan People’s Hospital, Tongchuan, ChinaInstitute of Oncology, Second Affiliated Hospital, Xi’an Medical University, Xi’an, ChinaInstitute of Oncology, Second Affiliated Hospital, Xi’an Medical University, Xi’an, ChinaPancreatic ductal adenocarcinoma (PDAC) poses a significant challenge in oncology due to its dismal prognosis and limited therapeutic options. In this study, we investigated the role of miR-301a in facilitating crosstalk between the Hedgehog (Hh) and HIPPO/YAP signaling pathways during the progression of PDAC. Our findings revealed that miR-301a served as a central regulatory node, targeting Gli1 within the Hh pathway and STK4 within the HIPPO/YAP pathway. Immunohistochemical and molecular analyses confirmed dysregulation of pathway components in pancreatic cancer, underscoring the pivotal role of miR-301a. Functional assays demonstrated the impact of miR-301a on cell proliferation and apoptosis, particularly in synergy with TNF-α. Overall, our study elucidated the intricate interplay between the Hh and HIPPO/YAP pathways mediated by miR-301a, providing valuable insights into potential therapeutic strategies for intervening in PDAC.https://www.tandfonline.com/doi/10.1080/15384047.2025.2457761miR-301aHedgehogGli1Hippo/YAPMST1(STK4) |
| spellingShingle | Bing Qi Yuqiong Wang Xian Zhu Yanfang Gong Jing Jin Hongyu Wu Xiaohua Man Feng Liu Wenzhu Yao Jun Gao miR-301a-mediated crosstalk between the Hedgehog and HIPPO/YAP signaling pathways promotes pancreatic cancer Cancer Biology & Therapy miR-301a Hedgehog Gli1 Hippo/YAP MST1(STK4) |
| title | miR-301a-mediated crosstalk between the Hedgehog and HIPPO/YAP signaling pathways promotes pancreatic cancer |
| title_full | miR-301a-mediated crosstalk between the Hedgehog and HIPPO/YAP signaling pathways promotes pancreatic cancer |
| title_fullStr | miR-301a-mediated crosstalk between the Hedgehog and HIPPO/YAP signaling pathways promotes pancreatic cancer |
| title_full_unstemmed | miR-301a-mediated crosstalk between the Hedgehog and HIPPO/YAP signaling pathways promotes pancreatic cancer |
| title_short | miR-301a-mediated crosstalk between the Hedgehog and HIPPO/YAP signaling pathways promotes pancreatic cancer |
| title_sort | mir 301a mediated crosstalk between the hedgehog and hippo yap signaling pathways promotes pancreatic cancer |
| topic | miR-301a Hedgehog Gli1 Hippo/YAP MST1(STK4) |
| url | https://www.tandfonline.com/doi/10.1080/15384047.2025.2457761 |
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