Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway
Objective: This study aimed to explore the cumulative effects and expression patterns of electroacupuncture (EA) on irisin secretion, observe the effects of EA on the recovery of neurobehavioral function and vascular remodeling after cerebral ischemia, and elucidate the mechanism by which EA promote...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Brain Research Bulletin |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923025000048 |
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| _version_ | 1825206990983397376 |
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| author | Lu Cao Wenchao Ding Yashuo Feng Chong Guan Li Liu Hongyu Xie Kewei Yu Dongyan Xu Lijuan Zhao Xuan Sha Xiaoman Deng Santian Wu Yangrui Wang Yi Wu Tingting Zhang Nianhong Wang |
| author_facet | Lu Cao Wenchao Ding Yashuo Feng Chong Guan Li Liu Hongyu Xie Kewei Yu Dongyan Xu Lijuan Zhao Xuan Sha Xiaoman Deng Santian Wu Yangrui Wang Yi Wu Tingting Zhang Nianhong Wang |
| author_sort | Lu Cao |
| collection | DOAJ |
| description | Objective: This study aimed to explore the cumulative effects and expression patterns of electroacupuncture (EA) on irisin secretion, observe the effects of EA on the recovery of neurobehavioral function and vascular remodeling after cerebral ischemia, and elucidate the mechanism by which EA promotes vascular remodeling by regulating irisin expression. Methods: A rat model of left middle cerebral artery occlusion (MCAO) was prepared, and EA was performed. Tissue distribution and expression of irisin were determined by immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and Western blotting. Type III fibronectin domain protein 5-silenced adeno-associated virus (rAAV-shFNDC5) was injected into the lateral ventricle as a control. Neurobehavioral function was evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) staining and behavioral experiments, while vascular remodeling was evaluated using laser speckle blood flow imaging, and the expressions of irisin and vascular remodeling-related factors were measured by ELISA and Western blotting. Results: The number of FNDC5-positive neurons, fluorescence intensity, and irisin expression reached their maximum increase after 7 days of EA treatment. In addition, the EA group exhibited a significant reduction in cerebral infarct volume and impairment of neurobehavioral function, an increase in cerebral blood flow and microvascular diameter on the ischemic side, and significantly higher expression levels of FNDC5, brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), protein kinase B (Akt), and endothelial nitric oxide synthase (eNOS). However, rAAV-shFNDC5 significantly weakened the therapeutic effects of EA. Conclusions: EA upregulated irisin expression, reaching a peak after 7 days of EA and then stabilizing. EA facilitated vascular remodeling after cerebral ischemia, and this might be associated with the activation of the irisin-mediated VEGF/Akt/eNOS signaling pathway. |
| format | Article |
| id | doaj-art-f1115827d8044ed3a7b11659555efd0f |
| institution | Kabale University |
| issn | 1873-2747 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj-art-f1115827d8044ed3a7b11659555efd0f2025-02-07T04:46:40ZengElsevierBrain Research Bulletin1873-27472025-02-01221111192Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathwayLu Cao0Wenchao Ding1Yashuo Feng2Chong Guan3Li Liu4Hongyu Xie5Kewei Yu6Dongyan Xu7Lijuan Zhao8Xuan Sha9Xiaoman Deng10Santian Wu11Yangrui Wang12Yi Wu13Tingting Zhang14Nianhong Wang15School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai, China; National Clinical Research Center for Geriatric Diseases, Huashan Hospital, Fudan University, Shanghai, China; Institute of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaSchool of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaSchool of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaSchool of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaSchool of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaSchool of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaSchool of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaLiving Word Shanghai, Shanghai, ChinaDepartment of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai, China; National Clinical Research Center for Geriatric Diseases, Huashan Hospital, Fudan University, Shanghai, ChinaSchool of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Correspondence to: School of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Department of Rehabilitation Medicine, Huashan Hospital, Fudan University, Shanghai, China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai, China; National Clinical Research Center for Geriatric Diseases, Huashan Hospital, Fudan University, Shanghai, China; Correspondence to: School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.Objective: This study aimed to explore the cumulative effects and expression patterns of electroacupuncture (EA) on irisin secretion, observe the effects of EA on the recovery of neurobehavioral function and vascular remodeling after cerebral ischemia, and elucidate the mechanism by which EA promotes vascular remodeling by regulating irisin expression. Methods: A rat model of left middle cerebral artery occlusion (MCAO) was prepared, and EA was performed. Tissue distribution and expression of irisin were determined by immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and Western blotting. Type III fibronectin domain protein 5-silenced adeno-associated virus (rAAV-shFNDC5) was injected into the lateral ventricle as a control. Neurobehavioral function was evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) staining and behavioral experiments, while vascular remodeling was evaluated using laser speckle blood flow imaging, and the expressions of irisin and vascular remodeling-related factors were measured by ELISA and Western blotting. Results: The number of FNDC5-positive neurons, fluorescence intensity, and irisin expression reached their maximum increase after 7 days of EA treatment. In addition, the EA group exhibited a significant reduction in cerebral infarct volume and impairment of neurobehavioral function, an increase in cerebral blood flow and microvascular diameter on the ischemic side, and significantly higher expression levels of FNDC5, brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), protein kinase B (Akt), and endothelial nitric oxide synthase (eNOS). However, rAAV-shFNDC5 significantly weakened the therapeutic effects of EA. Conclusions: EA upregulated irisin expression, reaching a peak after 7 days of EA and then stabilizing. EA facilitated vascular remodeling after cerebral ischemia, and this might be associated with the activation of the irisin-mediated VEGF/Akt/eNOS signaling pathway.http://www.sciencedirect.com/science/article/pii/S0361923025000048Ischemic strokeElectroacupunctureIrisinVascular remodeling |
| spellingShingle | Lu Cao Wenchao Ding Yashuo Feng Chong Guan Li Liu Hongyu Xie Kewei Yu Dongyan Xu Lijuan Zhao Xuan Sha Xiaoman Deng Santian Wu Yangrui Wang Yi Wu Tingting Zhang Nianhong Wang Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway Brain Research Bulletin Ischemic stroke Electroacupuncture Irisin Vascular remodeling |
| title | Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway |
| title_full | Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway |
| title_fullStr | Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway |
| title_full_unstemmed | Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway |
| title_short | Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway |
| title_sort | effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on vegf akt enos signaling pathway |
| topic | Ischemic stroke Electroacupuncture Irisin Vascular remodeling |
| url | http://www.sciencedirect.com/science/article/pii/S0361923025000048 |
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