Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera

Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera

Abstract Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool to discriminate fluorescent molecules or probe their nanoscale environment. Traditionally, FLIM uses time-correlated single-photon counting (TCSPC), which is precise but intrinsically low-throughput due to its dependence on...

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Main Authors: Nathan Ronceray, Salim Bennani, Marianna Fanouria Mitsioni, Nicole Siegel, Maria J. Marcaida, Claudio Bruschini, Edoardo Charbon, Rahul Roy, Matteo Dal Peraro, Guillermo P. Acuna, Aleksandra Radenovic
Format: Article
Language:English
Published: Nature Publishing Group 2025-08-01
Series:Light: Science & Applications
Online Access:https://doi.org/10.1038/s41377-025-01901-2
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https://doi.org/10.1038/s41377-025-01901-2

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