Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis
Mycobacterium tuberculosis the causative agent of tuberculosis affects millions of people worldwide. New tools for treatment and prevention of tuberculosis are urgently needed. We previously showed that a ferritin (bfrB) mutant of M. tuberculosis has altered iron homeostasis and increased sensitivit...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/385402 |
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| author | Selvakumar Subbian Ruchi Pandey Patricia Soteropoulos G. Marcela Rodriguez |
| author_facet | Selvakumar Subbian Ruchi Pandey Patricia Soteropoulos G. Marcela Rodriguez |
| author_sort | Selvakumar Subbian |
| collection | DOAJ |
| description | Mycobacterium tuberculosis the causative agent of tuberculosis affects millions of people worldwide. New tools for treatment and prevention of tuberculosis are urgently needed. We previously showed that a ferritin (bfrB) mutant of M. tuberculosis has altered iron homeostasis and increased sensitivity to antibiotics and to microbicidal effectors produced by activated macrophages. Most importantly, M. tuberculosis lacking BfrB is strongly attenuated in mice, especially, during the chronic phase of infection. In this study, we examined whether immunization with a bfrB mutant could confer protection against subsequent infection with virulent M. tuberculosis in a mouse model. The results show that the protection elicited by immunization with the bfrB mutant is comparable to BCG vaccination with respect to reduction of bacterial burden. However, significant distinctions in the disease pathology and host genome-wide lung transcriptome suggest improved containment of Mtb infection in animals vaccinated with the bfrB mutant, compared to BCG. We found that downmodulation of inflammatory response and enhanced fibrosis, compared to BCG vaccination, is associated with the protective response elicited by the bfrB mutant. |
| format | Article |
| id | doaj-art-ef1e0e68ce9642019180c8d1445a3c2d |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-ef1e0e68ce9642019180c8d1445a3c2d2025-02-03T06:07:32ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/385402385402Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosisSelvakumar Subbian0Ruchi Pandey1Patricia Soteropoulos2G. Marcela Rodriguez3Laboratory of Mycobacterial Immunity and Pathogenesis, Rutgers, The State University of New Jersey, 225 Warren Street, Newark, NJ 07103, USAPublic Health Research Institute at Rutgers Biomedical and Health Sciences, Rutgers, The State University of New Jersey, 225 Warren Street, Newark, NJ 07103, USAPublic Health Research Institute at Rutgers Biomedical and Health Sciences, Rutgers, The State University of New Jersey, 225 Warren Street, Newark, NJ 07103, USAPublic Health Research Institute at Rutgers Biomedical and Health Sciences, Rutgers, The State University of New Jersey, 225 Warren Street, Newark, NJ 07103, USAMycobacterium tuberculosis the causative agent of tuberculosis affects millions of people worldwide. New tools for treatment and prevention of tuberculosis are urgently needed. We previously showed that a ferritin (bfrB) mutant of M. tuberculosis has altered iron homeostasis and increased sensitivity to antibiotics and to microbicidal effectors produced by activated macrophages. Most importantly, M. tuberculosis lacking BfrB is strongly attenuated in mice, especially, during the chronic phase of infection. In this study, we examined whether immunization with a bfrB mutant could confer protection against subsequent infection with virulent M. tuberculosis in a mouse model. The results show that the protection elicited by immunization with the bfrB mutant is comparable to BCG vaccination with respect to reduction of bacterial burden. However, significant distinctions in the disease pathology and host genome-wide lung transcriptome suggest improved containment of Mtb infection in animals vaccinated with the bfrB mutant, compared to BCG. We found that downmodulation of inflammatory response and enhanced fibrosis, compared to BCG vaccination, is associated with the protective response elicited by the bfrB mutant.http://dx.doi.org/10.1155/2015/385402 |
| spellingShingle | Selvakumar Subbian Ruchi Pandey Patricia Soteropoulos G. Marcela Rodriguez Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis Journal of Immunology Research |
| title | Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis |
| title_full | Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis |
| title_fullStr | Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis |
| title_full_unstemmed | Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis |
| title_short | Vaccination with an Attenuated Ferritin Mutant Protects Mice against Virulent Mycobacterium tuberculosis |
| title_sort | vaccination with an attenuated ferritin mutant protects mice against virulent mycobacterium tuberculosis |
| url | http://dx.doi.org/10.1155/2015/385402 |
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