A survey on the off-target effects of insecticidal double-stranded RNA targeting the Hvβ´COPI gene in the crop pest Henosepilachna vigintioctopunctata through RNA-seq

A survey on the off-target effects of insecticidal double-stranded RNA targeting the Hvβ´COPI gene in the crop pest Henosepilachna vigintioctopunctata through RNA-seq

The specificity of the double-stranded RNA (dsRNA) used in the RNA interference (RNAi) technique is crucial for the success of sequence-specific gene silencing. Currently, RNAi-mediated insect control is a trending research topic. However, the off-target effects of the dsRNA in RNAi are a major conc...

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Bibliographic Details
Main Authors: Jing LÜ, Satyabrata NANDA, Shi-min CHEN, Yang MEI, Kang HE, Bao-li QIU, You-jun ZHANG, Fei LI, Hui-peng PAN
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2022-01-01
Series:Journal of Integrative Agriculture
Subjects:
RNAi
dsRNA
off-target effects
RNA-seq
transcriptome analysis
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311922000247
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