Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata

Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata

Abstract Cnidarians have become valuable models for understanding many aspects of developmental biology including the evolution of body plan diversity, novel cell type specification, and regeneration. Most of our understanding of gene function during early development in cnidarians comes from a smal...

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Bibliographic Details
Main Authors: Jacob F. Warner, Ryan Besemer, Alicia Schickle, Erin Borbee, Isabella V. Changsut, Koty Sharp, Leslie S. Babonis
Format: Article
Language:English
Published: BMC 2025-05-01
Series:EvoDevo
Subjects:
Functional genomics
Spawning
In vitro fertilization
Transgenesis
Scleractinia
Homology-directed repair
Online Access:https://doi.org/10.1186/s13227-025-00243-9
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https://doi.org/10.1186/s13227-025-00243-9

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