Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells
The expansion and maintenance of the NPMSC (nucleus pulposus mesenchymal stem cell) phenotype are considered as potential therapeutic tools for clinical applications in intervertebral disc tissue engineering and regenerative medicine. However, the harsh microenvironment within the intervertebral dis...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/1121064 |
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| _version_ | 1832562436169793536 |
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| author | Hua Zhang Wei Li YaoHong Wu Shengquan Zhang Jie Li Letian Han Haoyu Chen Ziyu Wang Cailiang Shen Yinshun Zhang Hui Tao |
| author_facet | Hua Zhang Wei Li YaoHong Wu Shengquan Zhang Jie Li Letian Han Haoyu Chen Ziyu Wang Cailiang Shen Yinshun Zhang Hui Tao |
| author_sort | Hua Zhang |
| collection | DOAJ |
| description | The expansion and maintenance of the NPMSC (nucleus pulposus mesenchymal stem cell) phenotype are considered as potential therapeutic tools for clinical applications in intervertebral disc tissue engineering and regenerative medicine. However, the harsh microenvironment within the intervertebral disc is the main limitation of its regeneration. The osmolarity of the intervertebral disc is higher than that of other tissues, which has an important influence on the biological characteristics of NPMSCs. In this study, we observed the effect of different osmolarities on the biological characteristics of human normal NPMSCs cultured in vitro and explored the role of osmolarity in intervertebral disc degeneration. Our data demonstrated that the change in osmotic pressure has an important effect on the biological activity of NPMSCs, and this effect may occur through the P16INK4A/Rb pathway. This study provides a theoretical basis for the future treatment of intervertebral disc degeneration. |
| format | Article |
| id | doaj-art-eea1ac1d32b643f2b85eede33d26646e |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-eea1ac1d32b643f2b85eede33d26646e2025-02-03T01:22:45ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/1121064Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem CellsHua Zhang0Wei Li1YaoHong Wu2Shengquan Zhang3Jie Li4Letian Han5Haoyu Chen6Ziyu Wang7Cailiang Shen8Yinshun Zhang9Hui Tao10Department of Orthopedics & Spine SurgeryDepartment of Orthopedics & Spine SurgeryDepartment of Spinal SurgeryDepartment of Biochemistry and Molecular BiologyDepartment of Clinical LaboratoryDepartment of Orthopedics & Spine SurgeryDepartment of Orthopedics & Spine SurgeryDepartment of Clinical LaboratoryDepartment of Orthopedics & Spine SurgeryDepartment of Orthopedics & Spine SurgeryDepartment of Orthopedics & Spine SurgeryThe expansion and maintenance of the NPMSC (nucleus pulposus mesenchymal stem cell) phenotype are considered as potential therapeutic tools for clinical applications in intervertebral disc tissue engineering and regenerative medicine. However, the harsh microenvironment within the intervertebral disc is the main limitation of its regeneration. The osmolarity of the intervertebral disc is higher than that of other tissues, which has an important influence on the biological characteristics of NPMSCs. In this study, we observed the effect of different osmolarities on the biological characteristics of human normal NPMSCs cultured in vitro and explored the role of osmolarity in intervertebral disc degeneration. Our data demonstrated that the change in osmotic pressure has an important effect on the biological activity of NPMSCs, and this effect may occur through the P16INK4A/Rb pathway. This study provides a theoretical basis for the future treatment of intervertebral disc degeneration.http://dx.doi.org/10.1155/2022/1121064 |
| spellingShingle | Hua Zhang Wei Li YaoHong Wu Shengquan Zhang Jie Li Letian Han Haoyu Chen Ziyu Wang Cailiang Shen Yinshun Zhang Hui Tao Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells Stem Cells International |
| title | Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells |
| title_full | Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells |
| title_fullStr | Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells |
| title_full_unstemmed | Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells |
| title_short | Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells |
| title_sort | effects of changes in osmolarity on the biological activity of human normal nucleus pulposus mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2022/1121064 |
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