Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

Abstract CRISPR/Cas12a, a promising gene editing technology, faces limitations due to its requirement for a thymine (T)‐rich protospacer adjacent motif (PAM). Despite the development of Cas12a variants with expanded PAM profiles, many genomic loci, especially those with guanine‐cytosine (GC)‐rich PA...

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Bibliographic Details
Main Authors: Zehua Chen, Junyuan Xue, Ziying Wang, Jinyuan Sun, Yinglu Cui, Tong Zhu, Huaiyi Yang, Ming Li, Bian Wu
Format: Article
Language:English
Published: Wiley 2025-08-01
Series:Advanced Science
Subjects:
CRISPR/Cas12a
expanded PAM profiles
gene editing
RNA toxin‐assisted evolution
Online Access:https://doi.org/10.1002/advs.202417105
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https://doi.org/10.1002/advs.202417105

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