Two-step cross-linking method for identification of NF-κB gene network by chromatin immunoprecipitation
The chromatin immunoprecipitation (ChIP) assay has recently been exploited as a powerful and versatile technique for probing protein-DNA interactions within the chromatin environment. In this method, intact cells are fixed with a reversible DNA-protein cross-linking agent (formaldehyde), and associa...
Saved in:
| Main Authors: | David E. Nowak, Bing Tian, Allan R. Brasier |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-11-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112014 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Protocol to investigate bivalent histone modification dynamics via chromatin immunoprecipitation followed by re-chromatin immunoprecipitation
by: Zhengyao Shao, et al.
Published: (2025-06-01) -
Magnetic Beads Make Chromatin Immunoprecipitation Faster and Easier
by: John Heyman, et al.
Published: (2007-05-01) -
Protocol for development and use of spike-in control for chromatin immunoprecipitation of chromatin-binding proteins in Schizosaccharomyces pombe
by: Jasbeer S. Khanduja, et al.
Published: (2025-09-01) -
Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins.
by: Nikolay A Pchelintsev, et al.
Published: (2016-01-01) -
Features of mammalian microRNA promoters emerge from polymerase II chromatin immunoprecipitation data.
by: David L Corcoran, et al.
Published: (2009-01-01)