Two-step cross-linking method for identification of NF-κB gene network by chromatin immunoprecipitation

The chromatin immunoprecipitation (ChIP) assay has recently been exploited as a powerful and versatile technique for probing protein-DNA interactions within the chromatin environment. In this method, intact cells are fixed with a reversible DNA-protein cross-linking agent (formaldehyde), and associa...

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Bibliographic Details
Main Authors: David E. Nowak, Bing Tian, Allan R. Brasier
Format: Article
Language:English
Published: Taylor & Francis Group 2005-11-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112014
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