Decreased number of myeloid-derived suppressor cells in the placental trophoblast of gestational diabetes mellitus. Possible role of leptin

Gestational diabetes mellitus (GDM) is the most common complication of pregnancy and significantly increases both maternal and fetal morbidity and mortality. Inflammation is a hallmark of GDM, and placental inflammation may play a key role in the pathophysiology of the disease. Myeloid-derived suppr...

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Main Authors: Malika Tami, Lourdes Hontecillas-Prieto, Daniel García-Domínguez, Rocío Flores-Campos, Teresa Vilariño-García, Flora Sánchez-Jiménez, Pilar Guadix, José L. Dueñas, Carlos Jiménez-Cortegana, Luis de la Cruz-Merino, Antonio Pérez-Pérez, Víctor Sánchez-Margalet
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Immunobiology
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Online Access:http://www.sciencedirect.com/science/article/pii/S0171298525000312
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author Malika Tami
Lourdes Hontecillas-Prieto
Daniel García-Domínguez
Rocío Flores-Campos
Teresa Vilariño-García
Flora Sánchez-Jiménez
Pilar Guadix
José L. Dueñas
Carlos Jiménez-Cortegana
Luis de la Cruz-Merino
Antonio Pérez-Pérez
Víctor Sánchez-Margalet
author_facet Malika Tami
Lourdes Hontecillas-Prieto
Daniel García-Domínguez
Rocío Flores-Campos
Teresa Vilariño-García
Flora Sánchez-Jiménez
Pilar Guadix
José L. Dueñas
Carlos Jiménez-Cortegana
Luis de la Cruz-Merino
Antonio Pérez-Pérez
Víctor Sánchez-Margalet
author_sort Malika Tami
collection DOAJ
description Gestational diabetes mellitus (GDM) is the most common complication of pregnancy and significantly increases both maternal and fetal morbidity and mortality. Inflammation is a hallmark of GDM, and placental inflammation may play a key role in the pathophysiology of the disease. Myeloid-derived suppressor cells (MDSCs), which are innate immunosuppressive, are thought to contribute to feto-maternal tolerance. In normal pregnancies, elevated levels of MDSCs have been observed in both peripheral and umbilical cord blood. Our hypothesis postulates that trophoblasts from placentas belonging to women with GDM may have lower levels of MDSCs compared to trophoblasts from placentas originating from healthy pregnancies. Furthermore, since leptin is overexpressed in the placenta of GDM patients, we hypothesized that leptin might contribute to the reduction of MDSCs. To test this, we investigated the in vitro effects of leptin on MDSC levels in isolated peripheral blood leukocytes after 24 h of incubation. Our findings indicate that trophoblasts from placentas from women with GDM contain a lower percentage of MDSCs compared to trophoblasts from healthy pregnancies. In addition, in vitro studies demonstrated that leptin reduces the number of MDSCs in peripheral blood leukocytes.In conclusion, MDSCs are decreased in placentas from pregnancies with GDM, and leptin appears to reduce the number of MDSCs in leukocytes isolated in vitro. Increased leptin expression in trophoblasts from placentas of women with GDM may contribute to the lower levels of MDSCs, potentially playing a role in placental inflammation. However, further investigations are required to fully elucidate this mechanism.
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spelling doaj-art-ed45be7edcc7472093a67cc6fef092b32025-08-20T02:03:14ZengElsevierImmunobiology0171-29852025-05-01230315289710.1016/j.imbio.2025.152897Decreased number of myeloid-derived suppressor cells in the placental trophoblast of gestational diabetes mellitus. Possible role of leptinMalika Tami0Lourdes Hontecillas-Prieto1Daniel García-Domínguez2Rocío Flores-Campos3Teresa Vilariño-García4Flora Sánchez-Jiménez5Pilar Guadix6José L. Dueñas7Carlos Jiménez-Cortegana8Luis de la Cruz-Merino9Antonio Pérez-Pérez10Víctor Sánchez-Margalet11Department of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, SpainDepartment of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, Spain; Institute of Biomedicine of Seville, Virgen Macarena University Hospital, CSIC, University of Seville, Seville, SpainDepartment of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, Spain; Institute of Biomedicine of Seville, Virgen Macarena University Hospital, CSIC, University of Seville, Seville, SpainDepartment of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, SpainDepartment of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, SpainDepartment of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, SpainService of Obstetrics and Gynecology, Virgen Macarena University Hospital. Medical, School, University of Seville, SpainService of Obstetrics and Gynecology, Virgen Macarena University Hospital. Medical, School, University of Seville, SpainDepartment of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, SpainInstitute of Biomedicine of Seville, Virgen Macarena University Hospital, CSIC, University of Seville, Seville, Spain; Medical Oncology Service, Department of Medicine, Medical School, Virgen Macarena, University Hospital, University of Seville, SpainDepartment of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, SpainDepartment of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School. Virgen Macarena University Hospital, University of Seville, Spain; Institute of Biomedicine of Seville, Virgen Macarena University Hospital, CSIC, University of Seville, Seville, Spain; Corresponding author at: Department of Medical Biochemistry and Molecular Biology, and Immunology, Medical, School Virgen Macarena University Hospital, University of Seville, Av. Dr. Fedriani 3, 41009 Seville, Spain.Gestational diabetes mellitus (GDM) is the most common complication of pregnancy and significantly increases both maternal and fetal morbidity and mortality. Inflammation is a hallmark of GDM, and placental inflammation may play a key role in the pathophysiology of the disease. Myeloid-derived suppressor cells (MDSCs), which are innate immunosuppressive, are thought to contribute to feto-maternal tolerance. In normal pregnancies, elevated levels of MDSCs have been observed in both peripheral and umbilical cord blood. Our hypothesis postulates that trophoblasts from placentas belonging to women with GDM may have lower levels of MDSCs compared to trophoblasts from placentas originating from healthy pregnancies. Furthermore, since leptin is overexpressed in the placenta of GDM patients, we hypothesized that leptin might contribute to the reduction of MDSCs. To test this, we investigated the in vitro effects of leptin on MDSC levels in isolated peripheral blood leukocytes after 24 h of incubation. Our findings indicate that trophoblasts from placentas from women with GDM contain a lower percentage of MDSCs compared to trophoblasts from healthy pregnancies. In addition, in vitro studies demonstrated that leptin reduces the number of MDSCs in peripheral blood leukocytes.In conclusion, MDSCs are decreased in placentas from pregnancies with GDM, and leptin appears to reduce the number of MDSCs in leukocytes isolated in vitro. Increased leptin expression in trophoblasts from placentas of women with GDM may contribute to the lower levels of MDSCs, potentially playing a role in placental inflammation. However, further investigations are required to fully elucidate this mechanism.http://www.sciencedirect.com/science/article/pii/S0171298525000312MDSCLeptinPlacentaGestational diabetes mellitus
spellingShingle Malika Tami
Lourdes Hontecillas-Prieto
Daniel García-Domínguez
Rocío Flores-Campos
Teresa Vilariño-García
Flora Sánchez-Jiménez
Pilar Guadix
José L. Dueñas
Carlos Jiménez-Cortegana
Luis de la Cruz-Merino
Antonio Pérez-Pérez
Víctor Sánchez-Margalet
Decreased number of myeloid-derived suppressor cells in the placental trophoblast of gestational diabetes mellitus. Possible role of leptin
Immunobiology
MDSC
Leptin
Placenta
Gestational diabetes mellitus
title Decreased number of myeloid-derived suppressor cells in the placental trophoblast of gestational diabetes mellitus. Possible role of leptin
title_full Decreased number of myeloid-derived suppressor cells in the placental trophoblast of gestational diabetes mellitus. Possible role of leptin
title_fullStr Decreased number of myeloid-derived suppressor cells in the placental trophoblast of gestational diabetes mellitus. Possible role of leptin
title_full_unstemmed Decreased number of myeloid-derived suppressor cells in the placental trophoblast of gestational diabetes mellitus. Possible role of leptin
title_short Decreased number of myeloid-derived suppressor cells in the placental trophoblast of gestational diabetes mellitus. Possible role of leptin
title_sort decreased number of myeloid derived suppressor cells in the placental trophoblast of gestational diabetes mellitus possible role of leptin
topic MDSC
Leptin
Placenta
Gestational diabetes mellitus
url http://www.sciencedirect.com/science/article/pii/S0171298525000312
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