Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groups

Abstract Water pollution by metals and metalloids promotes toxic effects to aquatic biota especially in mining regions. Environmental legislation applied to protect aquatic life from the toxicity of metals relies on the definition of protective values (PVs) for each compound. Among methods used to d...

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Main Authors: Gisele Alves Miranda, Fábio Soares dos Santos, Marcela Lourenço Pereira Cardoso, Matthew Etterson, Camila C. Amorim, Maria Clara V. M. Starling
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-92692-4
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author Gisele Alves Miranda
Fábio Soares dos Santos
Marcela Lourenço Pereira Cardoso
Matthew Etterson
Camila C. Amorim
Maria Clara V. M. Starling
author_facet Gisele Alves Miranda
Fábio Soares dos Santos
Marcela Lourenço Pereira Cardoso
Matthew Etterson
Camila C. Amorim
Maria Clara V. M. Starling
author_sort Gisele Alves Miranda
collection DOAJ
description Abstract Water pollution by metals and metalloids promotes toxic effects to aquatic biota especially in mining regions. Environmental legislation applied to protect aquatic life from the toxicity of metals relies on the definition of protective values (PVs) for each compound. Among methods used to define PVs, Species Sensitivity Distribution (SSD) curves enable the derivation of the Predicted No Effect concentration (PNEC). In this context, this is one of the first studies to propose the construction of acute and chronic split SSD curves built separately for three groups of freshwater organisms (algae, invertebrates and fish) to derive PNEC values for the 14 metals most commonly observed in iron ore mining tailings. Data used to construct split SSD curves were derived from the USEPA ECOTOX knowledgebase and EnviroTox databases and segregated according to the freshwater organism group and as “acute” or “chronic” toxicity. Then, split SSD curves were built using a minimum of nine species for each group to determine the hazardous concentration to 5% of species (HC5) and PNEC values for each group. Once PNEC were derived, a framework was proposed to calculate the Bioavailabity Factor (BioF) used to adjust values for local bioavailability conditions considering water quality characteristics in different regions. The lowest acute PNEC were observed for algae and invertebrates and corresponded to Silver (Ag). Nearly half of calculated PNEC were below current PVs in practice in Brazil, United States (US), United Kingdom (UK), Canada and European Union (EU). Results reinforce the pertinence of: (i) splitting SSD curves to define PVs for metals; and (ii) taking bioavailability into consideration to correct PNEC for local conditions. In addition, outcomes suggest that it is critical to rethink PVs related to metals for aquatic life protection, mainly in Brazil and Minas Gerais state, a region known for extensive mining activity. Finally, PNEC values obtained in this study may be used for ecological risk assessment studies, especially in areas affected by mining and other activities that result in pollution by metals and metalloids, such as Brazil.
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spelling doaj-art-ecc8598f691a4eafbcd768ca57ba68b82025-08-20T03:01:43ZengNature PortfolioScientific Reports2045-23222025-03-0115111310.1038/s41598-025-92692-4Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groupsGisele Alves Miranda0Fábio Soares dos Santos1Marcela Lourenço Pereira Cardoso2Matthew Etterson3Camila C. Amorim4Maria Clara V. M. Starling5Research Group on Environmental Applications of Advanced Oxidation Processes, Department of Sanitary and Environmental Engineering, The Federal University of Minas GeraisDepartment of Sanitary and Environmental Engineering, Federal University of Minas GeraisResearch Group on Environmental Applications of Advanced Oxidation Processes, Department of Sanitary and Environmental Engineering, The Federal University of Minas GeraisOffice of Research and Development, Center for Computational Toxicology and Exposure, Great Lakes Toxicology and Ecology Division, US Environmental Protection AgencyResearch Group on Environmental Applications of Advanced Oxidation Processes, Department of Sanitary and Environmental Engineering, The Federal University of Minas GeraisResearch Group on Environmental Applications of Advanced Oxidation Processes, Department of Sanitary and Environmental Engineering, The Federal University of Minas GeraisAbstract Water pollution by metals and metalloids promotes toxic effects to aquatic biota especially in mining regions. Environmental legislation applied to protect aquatic life from the toxicity of metals relies on the definition of protective values (PVs) for each compound. Among methods used to define PVs, Species Sensitivity Distribution (SSD) curves enable the derivation of the Predicted No Effect concentration (PNEC). In this context, this is one of the first studies to propose the construction of acute and chronic split SSD curves built separately for three groups of freshwater organisms (algae, invertebrates and fish) to derive PNEC values for the 14 metals most commonly observed in iron ore mining tailings. Data used to construct split SSD curves were derived from the USEPA ECOTOX knowledgebase and EnviroTox databases and segregated according to the freshwater organism group and as “acute” or “chronic” toxicity. Then, split SSD curves were built using a minimum of nine species for each group to determine the hazardous concentration to 5% of species (HC5) and PNEC values for each group. Once PNEC were derived, a framework was proposed to calculate the Bioavailabity Factor (BioF) used to adjust values for local bioavailability conditions considering water quality characteristics in different regions. The lowest acute PNEC were observed for algae and invertebrates and corresponded to Silver (Ag). Nearly half of calculated PNEC were below current PVs in practice in Brazil, United States (US), United Kingdom (UK), Canada and European Union (EU). Results reinforce the pertinence of: (i) splitting SSD curves to define PVs for metals; and (ii) taking bioavailability into consideration to correct PNEC for local conditions. In addition, outcomes suggest that it is critical to rethink PVs related to metals for aquatic life protection, mainly in Brazil and Minas Gerais state, a region known for extensive mining activity. Finally, PNEC values obtained in this study may be used for ecological risk assessment studies, especially in areas affected by mining and other activities that result in pollution by metals and metalloids, such as Brazil.https://doi.org/10.1038/s41598-025-92692-4EcotoxicityMiningBioavailabilityEnvironmental legislationAquatic life protection
spellingShingle Gisele Alves Miranda
Fábio Soares dos Santos
Marcela Lourenço Pereira Cardoso
Matthew Etterson
Camila C. Amorim
Maria Clara V. M. Starling
Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groups
Scientific Reports
Ecotoxicity
Mining
Bioavailability
Environmental legislation
Aquatic life protection
title Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groups
title_full Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groups
title_fullStr Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groups
title_full_unstemmed Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groups
title_short Proposal of novel Predicted No Effect Concentrations (PNEC) for metals in freshwater using Species Sensitivity Distribution for different taxonomic groups
title_sort proposal of novel predicted no effect concentrations pnec for metals in freshwater using species sensitivity distribution for different taxonomic groups
topic Ecotoxicity
Mining
Bioavailability
Environmental legislation
Aquatic life protection
url https://doi.org/10.1038/s41598-025-92692-4
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