Novel single-chain fragment variable antibody targeting plasma membrane epitopes on porcine Y-chromosome-bearing sperm

Novel single-chain fragment variable antibody targeting plasma membrane epitopes on porcine Y-chromosome-bearing sperm

Abstract The aim of this study was to construct and produce a single-chain fragment variable (scFv) antibody targeting the plasma membrane epitope on porcine Y-chromosome-bearing sperm (Y-sperm). The hybridoma cloned from 4D1-E8 exhibited the highest specificity, and Y-sperm was used to generate the...

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Bibliographic Details
Main Authors: Marninphan Thongkham, Surat Hongsibsong, Supamit Mekchay, Anucha Sathanawongs, Xue-Ming Cao, Zhen-Lin Xu, Korawan Sringarm
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
Subjects:
ScFv antibody
Porcine Y-sperm
Sexing sperm
Swine
Online Access:https://doi.org/10.1038/s41598-025-00587-1
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https://doi.org/10.1038/s41598-025-00587-1

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