Modular and efficient assembly of CRISPR vectors and construction of an apple gene editing efficiency evaluation system

Modular and efficient assembly of CRISPR vectors and construction of an apple gene editing efficiency evaluation system

[Objective] The CRISPR/Cas gene editing technology has demonstrated a broad application prospect for diverse applications in genetic improvement programs targeting apple (Malus domestica) and other perennial fruit crops. However, conventional gene editing systems exhibit critical limitations, includ...

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Main Authors: HOU Feifan, HUANG Chuanmi, HAO Pengbo, CONG Liu, BAI Tuanhui, SHI Jiangli, WANG Miaomiao, LIU Yu, ZHAO Yujie, WAN Ran, SHEN Yawen, ZHANG Hengtao, ZHANG Kunxi, ZHENG Xianbo, JIAO Jian
Format: Article
Language:zho
Published: Editorial Office of Journal of Fruit Science 2025-08-01
Series:Guoshu xuebao
Subjects:
malus domestica
crispr/cas system
modular vector
gene editing efficiency
transient transformation
Online Access:http://fruitsci.zzgss.cn/english/upload/down/month_2508/250820250816.pdf
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http://fruitsci.zzgss.cn/english/upload/down/month_2508/250820250816.pdf

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