Dietary Fiber Deficiency Accelerates Colitis in Mice in the Short Term Independent of Short-Chain Fatty Acids

It is believed that short-chain fatty acids (SCFAs)—the bacterial metabolites produced by the digestion of dietary fiber—potentially contribute to the prevention of colitis. However, this beneficial effect has not been conclusively proven. We thus attempted to verify this beneficial effect by examin...

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Main Authors: Shoma Kanda, Haruki Usuda, Sonoko Karino, Takayuki Okamoto, Tomomi Niibayashi, Takahisa Yano, Kohji Naora, Koichiro Wada
Format: Article
Language:English
Published: MDPI AG 2024-08-01
Series:Gastroenterology Insights
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Online Access:https://www.mdpi.com/2036-7422/15/3/52
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author Shoma Kanda
Haruki Usuda
Sonoko Karino
Takayuki Okamoto
Tomomi Niibayashi
Takahisa Yano
Kohji Naora
Koichiro Wada
author_facet Shoma Kanda
Haruki Usuda
Sonoko Karino
Takayuki Okamoto
Tomomi Niibayashi
Takahisa Yano
Kohji Naora
Koichiro Wada
author_sort Shoma Kanda
collection DOAJ
description It is believed that short-chain fatty acids (SCFAs)—the bacterial metabolites produced by the digestion of dietary fiber—potentially contribute to the prevention of colitis. However, this beneficial effect has not been conclusively proven. We thus attempted to verify this beneficial effect by examining whether colitis can be caused or worsened by the deficiency in dietary fiber in mice. We found that dextran sodium sulfate (DSS)-induced colitis was accelerated under a fiber-deficient condition, but the fiber deficiency itself did not provoke colitis. Moreover, episodes of diarrhea and epithelium damage in the large intestine were found upon analysis 24 h after the intervention. Unfortunately, these symptoms and tissue damage could not be ameliorated by administering SCFAs. On the other hand, a fiber-deficient condition increased the population of <i>Desulfovibrio</i> spp. and decreased the population of <i>Lactobaccillus</i> spp. regardless of the presence or absence of DSS upon analysis 24 h after the intervention. These results suggest that a deficiency in dietary fiber makes the intestinal environment irritable to colitis-inducing stimuli within the short term. This change does not appear to be related to the presence of SCFAs, but to the alteration of microbiota. Hence, a regular intake of dietary fiber is strongly recommended to avoid colitis and preserve intestinal health.
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spelling doaj-art-eb9fc4d9d0f84ad884bb17e10d190bc02025-08-20T01:55:31ZengMDPI AGGastroenterology Insights2036-74142036-74222024-08-0115373074310.3390/gastroent15030052Dietary Fiber Deficiency Accelerates Colitis in Mice in the Short Term Independent of Short-Chain Fatty AcidsShoma Kanda0Haruki Usuda1Sonoko Karino2Takayuki Okamoto3Tomomi Niibayashi4Takahisa Yano5Kohji Naora6Koichiro Wada7Department of Pharmacology, Faculty of Medicine, Shimane University, 89-1 Enya-cho, Izumo-City 693-8501, Shimane, JapanDepartment of Pharmacology, Faculty of Medicine, Shimane University, 89-1 Enya-cho, Izumo-City 693-8501, Shimane, JapanDepartment of Pharmacology, Faculty of Medicine, Shimane University, 89-1 Enya-cho, Izumo-City 693-8501, Shimane, JapanDepartment of Pharmacology, Faculty of Medicine, Shimane University, 89-1 Enya-cho, Izumo-City 693-8501, Shimane, JapanDepartment of Pharmacology, Faculty of Medicine, Shimane University, 89-1 Enya-cho, Izumo-City 693-8501, Shimane, JapanDepartment of Pharmacy, Shimane University Hospital, 89-1 Enya-cho, Izumo-City 693-8501, Shimane, JapanDepartment of Pharmacy, Shimane University Hospital, 89-1 Enya-cho, Izumo-City 693-8501, Shimane, JapanDepartment of Pharmacology, Faculty of Medicine, Shimane University, 89-1 Enya-cho, Izumo-City 693-8501, Shimane, JapanIt is believed that short-chain fatty acids (SCFAs)—the bacterial metabolites produced by the digestion of dietary fiber—potentially contribute to the prevention of colitis. However, this beneficial effect has not been conclusively proven. We thus attempted to verify this beneficial effect by examining whether colitis can be caused or worsened by the deficiency in dietary fiber in mice. We found that dextran sodium sulfate (DSS)-induced colitis was accelerated under a fiber-deficient condition, but the fiber deficiency itself did not provoke colitis. Moreover, episodes of diarrhea and epithelium damage in the large intestine were found upon analysis 24 h after the intervention. Unfortunately, these symptoms and tissue damage could not be ameliorated by administering SCFAs. On the other hand, a fiber-deficient condition increased the population of <i>Desulfovibrio</i> spp. and decreased the population of <i>Lactobaccillus</i> spp. regardless of the presence or absence of DSS upon analysis 24 h after the intervention. These results suggest that a deficiency in dietary fiber makes the intestinal environment irritable to colitis-inducing stimuli within the short term. This change does not appear to be related to the presence of SCFAs, but to the alteration of microbiota. Hence, a regular intake of dietary fiber is strongly recommended to avoid colitis and preserve intestinal health.https://www.mdpi.com/2036-7422/15/3/52dietary fibercolitisshort-chain fatty acidsblood stoolmyeloperoxidase activityepithelial damage
spellingShingle Shoma Kanda
Haruki Usuda
Sonoko Karino
Takayuki Okamoto
Tomomi Niibayashi
Takahisa Yano
Kohji Naora
Koichiro Wada
Dietary Fiber Deficiency Accelerates Colitis in Mice in the Short Term Independent of Short-Chain Fatty Acids
Gastroenterology Insights
dietary fiber
colitis
short-chain fatty acids
blood stool
myeloperoxidase activity
epithelial damage
title Dietary Fiber Deficiency Accelerates Colitis in Mice in the Short Term Independent of Short-Chain Fatty Acids
title_full Dietary Fiber Deficiency Accelerates Colitis in Mice in the Short Term Independent of Short-Chain Fatty Acids
title_fullStr Dietary Fiber Deficiency Accelerates Colitis in Mice in the Short Term Independent of Short-Chain Fatty Acids
title_full_unstemmed Dietary Fiber Deficiency Accelerates Colitis in Mice in the Short Term Independent of Short-Chain Fatty Acids
title_short Dietary Fiber Deficiency Accelerates Colitis in Mice in the Short Term Independent of Short-Chain Fatty Acids
title_sort dietary fiber deficiency accelerates colitis in mice in the short term independent of short chain fatty acids
topic dietary fiber
colitis
short-chain fatty acids
blood stool
myeloperoxidase activity
epithelial damage
url https://www.mdpi.com/2036-7422/15/3/52
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