Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome
Background. The myelodysplastic syndrome (MDS) comprises a group of clonal hematopoietic stem cell diseases characterized by cytopenia, dysplasia in one or more of the major myeloid lineages, ineffective hematopoiesis, and increased risk of development of acute myeloid leukemia (AML). Macrophages ar...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/5479013 |
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| _version_ | 1832567415095951360 |
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| author | Yu Han Huaquan Wang Zonghong Shao |
| author_facet | Yu Han Huaquan Wang Zonghong Shao |
| author_sort | Yu Han |
| collection | DOAJ |
| description | Background. The myelodysplastic syndrome (MDS) comprises a group of clonal hematopoietic stem cell diseases characterized by cytopenia, dysplasia in one or more of the major myeloid lineages, ineffective hematopoiesis, and increased risk of development of acute myeloid leukemia (AML). Macrophages are innate immune cells that ingest and degrade abnormal cells, debris, and foreign material and orchestrate inflammatory processes. We analyzed the role of macrophages from MDS patients in vitro. Methods. Macrophages were induced from peripheral blood of patients with MDS via granulocyte macrophage colony-stimulating factor (GM-CSF). Phagocytic capacity of macrophages was measured with carboxyfluorescein succinimidyl ester and fluorescent microspheres. CD206 and signal regulatory protein alpha (SIRPα) on macrophages were detected by flow cytometry. Inducible nitric oxide synthase (iNOS) was measured by ELISA method. Results. Compared with normal control group, the number of monocytes increased in MDS patients. However, the monocytes showed impaired ability to induce macrophages and the number of macrophages induced from MDS samples was lower. Further, we demonstrated that the ex vivo phagocytic function of macrophages from MDS patients was impaired and levels of reorganization receptors CD206 and SIRPα were lower. Levels of iNOS secreted by macrophages in MDS were increased. Conclusions. Monocyte-derived macrophages are impaired in myelodysplastic syndromes. |
| format | Article |
| id | doaj-art-eb72d024f37e45a687caea2dfb6c43d2 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-eb72d024f37e45a687caea2dfb6c43d22025-02-03T01:01:37ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/54790135479013Monocyte-Derived Macrophages Are Impaired in Myelodysplastic SyndromeYu Han0Huaquan Wang1Zonghong Shao2Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, ChinaDepartment of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, ChinaDepartment of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, ChinaBackground. The myelodysplastic syndrome (MDS) comprises a group of clonal hematopoietic stem cell diseases characterized by cytopenia, dysplasia in one or more of the major myeloid lineages, ineffective hematopoiesis, and increased risk of development of acute myeloid leukemia (AML). Macrophages are innate immune cells that ingest and degrade abnormal cells, debris, and foreign material and orchestrate inflammatory processes. We analyzed the role of macrophages from MDS patients in vitro. Methods. Macrophages were induced from peripheral blood of patients with MDS via granulocyte macrophage colony-stimulating factor (GM-CSF). Phagocytic capacity of macrophages was measured with carboxyfluorescein succinimidyl ester and fluorescent microspheres. CD206 and signal regulatory protein alpha (SIRPα) on macrophages were detected by flow cytometry. Inducible nitric oxide synthase (iNOS) was measured by ELISA method. Results. Compared with normal control group, the number of monocytes increased in MDS patients. However, the monocytes showed impaired ability to induce macrophages and the number of macrophages induced from MDS samples was lower. Further, we demonstrated that the ex vivo phagocytic function of macrophages from MDS patients was impaired and levels of reorganization receptors CD206 and SIRPα were lower. Levels of iNOS secreted by macrophages in MDS were increased. Conclusions. Monocyte-derived macrophages are impaired in myelodysplastic syndromes.http://dx.doi.org/10.1155/2016/5479013 |
| spellingShingle | Yu Han Huaquan Wang Zonghong Shao Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome Journal of Immunology Research |
| title | Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome |
| title_full | Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome |
| title_fullStr | Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome |
| title_full_unstemmed | Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome |
| title_short | Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome |
| title_sort | monocyte derived macrophages are impaired in myelodysplastic syndrome |
| url | http://dx.doi.org/10.1155/2016/5479013 |
| work_keys_str_mv | AT yuhan monocytederivedmacrophagesareimpairedinmyelodysplasticsyndrome AT huaquanwang monocytederivedmacrophagesareimpairedinmyelodysplasticsyndrome AT zonghongshao monocytederivedmacrophagesareimpairedinmyelodysplasticsyndrome |