Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome

Background. The myelodysplastic syndrome (MDS) comprises a group of clonal hematopoietic stem cell diseases characterized by cytopenia, dysplasia in one or more of the major myeloid lineages, ineffective hematopoiesis, and increased risk of development of acute myeloid leukemia (AML). Macrophages ar...

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Main Authors: Yu Han, Huaquan Wang, Zonghong Shao
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2016/5479013
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author Yu Han
Huaquan Wang
Zonghong Shao
author_facet Yu Han
Huaquan Wang
Zonghong Shao
author_sort Yu Han
collection DOAJ
description Background. The myelodysplastic syndrome (MDS) comprises a group of clonal hematopoietic stem cell diseases characterized by cytopenia, dysplasia in one or more of the major myeloid lineages, ineffective hematopoiesis, and increased risk of development of acute myeloid leukemia (AML). Macrophages are innate immune cells that ingest and degrade abnormal cells, debris, and foreign material and orchestrate inflammatory processes. We analyzed the role of macrophages from MDS patients in vitro. Methods. Macrophages were induced from peripheral blood of patients with MDS via granulocyte macrophage colony-stimulating factor (GM-CSF). Phagocytic capacity of macrophages was measured with carboxyfluorescein succinimidyl ester and fluorescent microspheres. CD206 and signal regulatory protein alpha (SIRPα) on macrophages were detected by flow cytometry. Inducible nitric oxide synthase (iNOS) was measured by ELISA method. Results. Compared with normal control group, the number of monocytes increased in MDS patients. However, the monocytes showed impaired ability to induce macrophages and the number of macrophages induced from MDS samples was lower. Further, we demonstrated that the ex vivo phagocytic function of macrophages from MDS patients was impaired and levels of reorganization receptors CD206 and SIRPα were lower. Levels of iNOS secreted by macrophages in MDS were increased. Conclusions. Monocyte-derived macrophages are impaired in myelodysplastic syndromes.
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spelling doaj-art-eb72d024f37e45a687caea2dfb6c43d22025-02-03T01:01:37ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/54790135479013Monocyte-Derived Macrophages Are Impaired in Myelodysplastic SyndromeYu Han0Huaquan Wang1Zonghong Shao2Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, ChinaDepartment of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, ChinaDepartment of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, ChinaBackground. The myelodysplastic syndrome (MDS) comprises a group of clonal hematopoietic stem cell diseases characterized by cytopenia, dysplasia in one or more of the major myeloid lineages, ineffective hematopoiesis, and increased risk of development of acute myeloid leukemia (AML). Macrophages are innate immune cells that ingest and degrade abnormal cells, debris, and foreign material and orchestrate inflammatory processes. We analyzed the role of macrophages from MDS patients in vitro. Methods. Macrophages were induced from peripheral blood of patients with MDS via granulocyte macrophage colony-stimulating factor (GM-CSF). Phagocytic capacity of macrophages was measured with carboxyfluorescein succinimidyl ester and fluorescent microspheres. CD206 and signal regulatory protein alpha (SIRPα) on macrophages were detected by flow cytometry. Inducible nitric oxide synthase (iNOS) was measured by ELISA method. Results. Compared with normal control group, the number of monocytes increased in MDS patients. However, the monocytes showed impaired ability to induce macrophages and the number of macrophages induced from MDS samples was lower. Further, we demonstrated that the ex vivo phagocytic function of macrophages from MDS patients was impaired and levels of reorganization receptors CD206 and SIRPα were lower. Levels of iNOS secreted by macrophages in MDS were increased. Conclusions. Monocyte-derived macrophages are impaired in myelodysplastic syndromes.http://dx.doi.org/10.1155/2016/5479013
spellingShingle Yu Han
Huaquan Wang
Zonghong Shao
Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome
Journal of Immunology Research
title Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome
title_full Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome
title_fullStr Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome
title_full_unstemmed Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome
title_short Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome
title_sort monocyte derived macrophages are impaired in myelodysplastic syndrome
url http://dx.doi.org/10.1155/2016/5479013
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AT huaquanwang monocytederivedmacrophagesareimpairedinmyelodysplasticsyndrome
AT zonghongshao monocytederivedmacrophagesareimpairedinmyelodysplasticsyndrome