Pathological findings in rainbow trout (oncorhynchus mykiss, walbaum 1792) in experimental vagococcus salmoninarum infection
<b>Aim:</b> The aim of this study was to induce Vagococcus salmoninarum (V. salmoninarum) experimental infection in adult fish through different routes [intraperitoneal (IP) and immersion (IM)] to investigate clinical, macroscopical and histopathological findings.<p> <b>Mater...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1155 |
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| Summary: | <b>Aim:</b> The aim of this study was to induce Vagococcus salmoninarum (V. salmoninarum)
experimental infection in adult fish through different routes [intraperitoneal
(IP) and immersion (IM)] to investigate clinical, macroscopical and
histopathological findings.<p>
<b>Materials and Methods:</b> Totally 4 groups (experiment immersion-EIM, experiment
peritoneal-EIP and 2 controls) were used. As material, overall 60
rainbow trout were used. Fishes were infected with Vagococcus salmoninarum
(accession number: Kj685901) strain. For histopathological examinations,
all tissue samples were fixed and than fixation samples routinely were processed.
Tissue sections stained with haematoxylin-eosin and examined using
light microscope. DNA extractions of the isolate were made for the molecular
analysis of the bacteria and then, specific primers were used to identify V.
salmoninarum. The PCR product from isolate V. salmoninarum was used for
sequencing.<p>
<b>Results:</b> In both groups, mortality rate was 100%. In macroscopic examination,
abdominal swelling, depigmentation of the skin, hemorrhages at the
base of the fins, swelling in kidneys and a thick and yellow-orange fluid in the
intestine were seen. These findings were observed in both groups, but were
more severe in EIP group than in EIM group. In histopathologic examination,
the course of the disease ranged from acute form to chronic form in both groups.
In acute form, haemorrhage and telangiectasia in the gills and increase in
melanomacrophage centers were detected. In chronic form, degeneration and
necrosis in the kidney and epicarditis in three cases (EIP) were observed. Elevated
numbers and degranulation of eosinophilic granular cells (EGC) in the
submucosa of the digestive system were also observed. Degeneration was seen
in gastric epithelium and shedding of intestinal mucosa epithelium.<p>
<b>Conclusion:</b> In this study, approachs of V. salmoninarum infection in adult rainbow
trout, the differences between the findings in experimental infection
and natural infection is recommended to be taken into account. |
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| ISSN: | 1309-6958 2146-1953 |