Direct comparison of an ultrasensitive real-time PCR assay with droplet digital PCR for the detection of PIK3CA hotspot mutations in primary tumors, plasma cell-free DNA and paired CTC-derived gDNAs

Direct comparison of an ultrasensitive real-time PCR assay with droplet digital PCR for the detection of PIK3CA hotspot mutations in primary tumors, plasma cell-free DNA and paired CTC-derived gDNAs

IntroductionDetection of PIK3CA mutations in primary tumors and liquid biopsy samples is of increasing importance for treatment decisions and therapy resistance in many types of cancer. The aim of the present study was to directly compare the efficacy of a relatively inexpensive ultrasensitive real-...

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Main Authors: Stavroula Smilkou, Loukas Kaklamanis, Ioanna Balgouranidou, Helena Linardou, Alkistis Maria Papatheodoridi, Flora Zagouri, Evangelia Razis, Stylianos Kakolyris, Amanda Psyrri, Christos Papadimitriou, Athina Markou, Evi Lianidou
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Oncology
Subjects:
PIK3CA mutations
liquid biopsy
breast cancer
plasma cell-free DNA
circulating tumor cells
DNA melting curve analysis
Online Access:https://www.frontiersin.org/articles/10.3389/fonc.2024.1435559/full
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https://www.frontiersin.org/articles/10.3389/fonc.2024.1435559/full

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