Functionalized Polymeric Microneedles for Transdermal Delivery of Ovalbumin Protein Antigen
<b>Background/Objectives:</b> Microneedles represent an innovative transdermal drug delivery approach, especially for protein antigens. This study aimed to develop a dual-functional, dissolvable microneedle system loaded with β-glucan and fucoidan in a hyaluronic acid matrix to achieve t...
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2025-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/17/6/737 |
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| author | Yi Liu Feng Tan Decheng Zhao Liwen Zhang Nianni Zhang Chengwei Bai Ziyang Guo Xiongjian Guan Guanyu Chen |
| author_facet | Yi Liu Feng Tan Decheng Zhao Liwen Zhang Nianni Zhang Chengwei Bai Ziyang Guo Xiongjian Guan Guanyu Chen |
| author_sort | Yi Liu |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Microneedles represent an innovative transdermal drug delivery approach, especially for protein antigens. This study aimed to develop a dual-functional, dissolvable microneedle system loaded with β-glucan and fucoidan in a hyaluronic acid matrix to achieve transdermal immunomodulation and reactive oxygen species (ROS) regulation, exploring its potential in inflammatory disease management and antigen delivery. <b>Methods:</b> The microneedles were fabricated using a two-step casting method. Their morphology, mechanical strength, and dissolution kinetics were characterized. In vitro experiments evaluated the ROS-modulating effects on human dermal fibroblasts, while in vivo studies on C57 mice investigated immune activation and lymph node accumulation of ovalbumin antigen. <b>Results:</b> The microneedles exhibited a mechanical strength exceeding 7.45 N/needle and dissolved within 50 s. β-glucan transiently reduced ROS levels at 6 h followed by a rebound, whereas fucoidan sustained ROS suppression after 12 h. In mice, β-glucan-loaded microneedles triggered local immune activation, and fucoidan-incorporated microneedles enhanced ovalbumin accumulation in lymph nodes by 2.1-fold compared to controls. <b>Conclusions:</b> Integrating β-glucan’s immunostimulatory and fucoidan’s ROS-scavenging/lymphatic-targeting properties within a single microneedle platform offers a promising multifunctional strategy for treating inflammatory diseases and delivering protein antigens. |
| format | Article |
| id | doaj-art-e8354a399c5a49b48ff12ccc169ddafd |
| institution | Kabale University |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-e8354a399c5a49b48ff12ccc169ddafd2025-08-20T03:29:39ZengMDPI AGPharmaceutics1999-49232025-06-0117673710.3390/pharmaceutics17060737Functionalized Polymeric Microneedles for Transdermal Delivery of Ovalbumin Protein AntigenYi Liu0Feng Tan1Decheng Zhao2Liwen Zhang3Nianni Zhang4Chengwei Bai5Ziyang Guo6Xiongjian Guan7Guanyu Chen8School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University Shenzhen Campus, Shenzhen 518107, China<b>Background/Objectives:</b> Microneedles represent an innovative transdermal drug delivery approach, especially for protein antigens. This study aimed to develop a dual-functional, dissolvable microneedle system loaded with β-glucan and fucoidan in a hyaluronic acid matrix to achieve transdermal immunomodulation and reactive oxygen species (ROS) regulation, exploring its potential in inflammatory disease management and antigen delivery. <b>Methods:</b> The microneedles were fabricated using a two-step casting method. Their morphology, mechanical strength, and dissolution kinetics were characterized. In vitro experiments evaluated the ROS-modulating effects on human dermal fibroblasts, while in vivo studies on C57 mice investigated immune activation and lymph node accumulation of ovalbumin antigen. <b>Results:</b> The microneedles exhibited a mechanical strength exceeding 7.45 N/needle and dissolved within 50 s. β-glucan transiently reduced ROS levels at 6 h followed by a rebound, whereas fucoidan sustained ROS suppression after 12 h. In mice, β-glucan-loaded microneedles triggered local immune activation, and fucoidan-incorporated microneedles enhanced ovalbumin accumulation in lymph nodes by 2.1-fold compared to controls. <b>Conclusions:</b> Integrating β-glucan’s immunostimulatory and fucoidan’s ROS-scavenging/lymphatic-targeting properties within a single microneedle platform offers a promising multifunctional strategy for treating inflammatory diseases and delivering protein antigens.https://www.mdpi.com/1999-4923/17/6/737dissolvable microneedlestransdermal drug deliveryimmunomodulationreactive oxygen species (ROS) regulationβ-glucanfucoidan |
| spellingShingle | Yi Liu Feng Tan Decheng Zhao Liwen Zhang Nianni Zhang Chengwei Bai Ziyang Guo Xiongjian Guan Guanyu Chen Functionalized Polymeric Microneedles for Transdermal Delivery of Ovalbumin Protein Antigen Pharmaceutics dissolvable microneedles transdermal drug delivery immunomodulation reactive oxygen species (ROS) regulation β-glucan fucoidan |
| title | Functionalized Polymeric Microneedles for Transdermal Delivery of Ovalbumin Protein Antigen |
| title_full | Functionalized Polymeric Microneedles for Transdermal Delivery of Ovalbumin Protein Antigen |
| title_fullStr | Functionalized Polymeric Microneedles for Transdermal Delivery of Ovalbumin Protein Antigen |
| title_full_unstemmed | Functionalized Polymeric Microneedles for Transdermal Delivery of Ovalbumin Protein Antigen |
| title_short | Functionalized Polymeric Microneedles for Transdermal Delivery of Ovalbumin Protein Antigen |
| title_sort | functionalized polymeric microneedles for transdermal delivery of ovalbumin protein antigen |
| topic | dissolvable microneedles transdermal drug delivery immunomodulation reactive oxygen species (ROS) regulation β-glucan fucoidan |
| url | https://www.mdpi.com/1999-4923/17/6/737 |
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