Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS
Objectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination o...
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| Format: | Article |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/571201 |
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| author | Mustafa Karapirli Murat Kizilgun Ozgur Yesilyurt Husamettin Gul Zeki Ilker Kunak Emin Ozgur Akgul Enis Macit Tuncer Cayci Yasemin Gulcan Kurt Ibrahim Aydin Hakan Yaren Melik Seyrek Erdinc Cakir Halil Yaman |
| author_facet | Mustafa Karapirli Murat Kizilgun Ozgur Yesilyurt Husamettin Gul Zeki Ilker Kunak Emin Ozgur Akgul Enis Macit Tuncer Cayci Yasemin Gulcan Kurt Ibrahim Aydin Hakan Yaren Melik Seyrek Erdinc Cakir Halil Yaman |
| author_sort | Mustafa Karapirli |
| collection | DOAJ |
| description | Objectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of CyA, TRL, SIR, and RAD in whole-blood samples. Materials and Methods. We used an integrated online solid-phase extraction-LC-MS/MS system and atmospheric pressure ionization tandem mass spectrometry (API-MS/MS) in the multiple reaction monitoring (MRM) detection mode. CyA, TRL, SIR, and RAD were simultaneously analyzed in whole blood treated with precipitation reagent taken from transplant patients. Results. System performance parameters were suitable for using this method as a high-throughput technique in clinical practice. The high concentration of one analyte in the sample did not affect the concentration of other analytes. Total analytical time was 2.5 min, and retention times of all analytes were shorter than 2 minutes. Conclusion. This LC-MS/MS method can be preferable for therapeutic drug monitoring of these immunosuppressive drugs (CyA, TRL, SRL, and RAD) in whole blood. Sample preparation was too short and simple in this method, and it permits robust, rapid, sensitive, selective, and simultaneous determination of these drugs. |
| format | Article |
| id | doaj-art-e7b5e1ee5da9440897004eefc4ecb925 |
| institution | DOAJ |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e7b5e1ee5da9440897004eefc4ecb9252025-08-20T03:21:02ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/571201571201Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MSMustafa Karapirli0Murat Kizilgun1Ozgur Yesilyurt2Husamettin Gul3Zeki Ilker Kunak4Emin Ozgur Akgul5Enis Macit6Tuncer Cayci7Yasemin Gulcan Kurt8Ibrahim Aydin9Hakan Yaren10Melik Seyrek11Erdinc Cakir12Halil Yaman13Council of Forensic Medicine, Ankara Branch, Kecioren, 06018 Ankara, TurkeyDepartment of Biochemistry, Diskapi Children' Health and Diseases, Hematology, Oncology Training and Research Hospital, Ministry of Health, 06590 Ankara, TurkeyDepartment of Pharmacology, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Pharmacology, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Medical Chemical/Biological/Radiological/Nuclear Defense, Gulhane Military Medical Academy, Etlik, 06018 Ankara, TurkeyDepartment of Clinical Biochemistry, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Pharmacology, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Clinical Biochemistry, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Clinical Biochemistry, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Clinical Biochemistry, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Medical Chemical/Biological/Radiological/Nuclear Defense, Gulhane Military Medical Academy, Etlik, 06018 Ankara, TurkeyDepartment of Pharmacology, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Clinical Biochemistry, Gulhane Military Medical Academy, 06018 Ankara, TurkeyDepartment of Clinical Biochemistry, Gulhane Military Medical Academy, 06018 Ankara, TurkeyObjectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of CyA, TRL, SIR, and RAD in whole-blood samples. Materials and Methods. We used an integrated online solid-phase extraction-LC-MS/MS system and atmospheric pressure ionization tandem mass spectrometry (API-MS/MS) in the multiple reaction monitoring (MRM) detection mode. CyA, TRL, SIR, and RAD were simultaneously analyzed in whole blood treated with precipitation reagent taken from transplant patients. Results. System performance parameters were suitable for using this method as a high-throughput technique in clinical practice. The high concentration of one analyte in the sample did not affect the concentration of other analytes. Total analytical time was 2.5 min, and retention times of all analytes were shorter than 2 minutes. Conclusion. This LC-MS/MS method can be preferable for therapeutic drug monitoring of these immunosuppressive drugs (CyA, TRL, SRL, and RAD) in whole blood. Sample preparation was too short and simple in this method, and it permits robust, rapid, sensitive, selective, and simultaneous determination of these drugs.http://dx.doi.org/10.1100/2012/571201 |
| spellingShingle | Mustafa Karapirli Murat Kizilgun Ozgur Yesilyurt Husamettin Gul Zeki Ilker Kunak Emin Ozgur Akgul Enis Macit Tuncer Cayci Yasemin Gulcan Kurt Ibrahim Aydin Hakan Yaren Melik Seyrek Erdinc Cakir Halil Yaman Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS The Scientific World Journal |
| title | Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS |
| title_full | Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS |
| title_fullStr | Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS |
| title_full_unstemmed | Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS |
| title_short | Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS |
| title_sort | simultaneous determination of cyclosporine a tacrolimus sirolimus and everolimus in whole blood samples by lc ms ms |
| url | http://dx.doi.org/10.1100/2012/571201 |
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