Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium
Duck hepatitis B virus (DHBV) shares many basic characteristics with hepatitis B virus (HBV) and is an attractive model for vaccine development. In this study, DHBV DNA vaccines were designed to express envelope and capsid fusion proteins to enhance the breadth of immune response in ducks. Attenuate...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2018-04-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209531191761829X |
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| author | Si-yang LIU Ren-yong JIA Qing-qing LI Dai-shen FENG Hao-yue SHEN Cui YANG Ming-shu WANG De-kang ZHU Shun CHEN Ma-feng LIU Xin-xin ZHAO Zhong-qiong YIN Bo JING An-chun CHENG |
| author_facet | Si-yang LIU Ren-yong JIA Qing-qing LI Dai-shen FENG Hao-yue SHEN Cui YANG Ming-shu WANG De-kang ZHU Shun CHEN Ma-feng LIU Xin-xin ZHAO Zhong-qiong YIN Bo JING An-chun CHENG |
| author_sort | Si-yang LIU |
| collection | DOAJ |
| description | Duck hepatitis B virus (DHBV) shares many basic characteristics with hepatitis B virus (HBV) and is an attractive model for vaccine development. In this study, DHBV DNA vaccines were designed to express envelope and capsid fusion proteins to enhance the breadth of immune response in ducks. Attenuated Salmonella typhimurium (SL7207) was used as a carrier and adjuvant to boost the magnitude of immune response. Based on this strategy, novel DNA vaccines (SL7207-pVAX1-LC and SL7207-pVAX1-SC) were generated. Growth kinetics, genetic stabilities and relative transcription levels of the L, S and C genes introduced by these vaccine strains were measured before inoculation to guarantee safety and efficacy. The relative transcript levels of the CD4 and CD8 T genes and the antibody levels (IgY) in ducks receiving the vaccines were higher than those in single gene delivered groups. Additionally, the copy number of covalently closed circular DNA in hepatocytes after DHBV challenge also provided evidence that our fusion vaccines could enhance the protective efficiency against DHBV infection in ducks. |
| format | Article |
| id | doaj-art-e79a74ecf9904ca48b873d0d27913494 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e79a74ecf9904ca48b873d0d279134942025-08-20T03:58:03ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-04-0117492893910.1016/S2095-3119(17)61829-XImmunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimuriumSi-yang LIU0Ren-yong JIA1Qing-qing LI2Dai-shen FENG3Hao-yue SHEN4Cui YANG5Ming-shu WANG6De-kang ZHU7Shun CHEN8Ma-feng LIU9Xin-xin ZHAO10Zhong-qiong YIN11Bo JING12An-chun CHENG13Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. China; Correspondence JIA Ren-yong, Tel/Fax: +86-28-86291176Avian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaAvian Disease Research Center, Sichuan Agricultural University, Chengdu 611130, P.R. China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, P.R. ChinaDuck hepatitis B virus (DHBV) shares many basic characteristics with hepatitis B virus (HBV) and is an attractive model for vaccine development. In this study, DHBV DNA vaccines were designed to express envelope and capsid fusion proteins to enhance the breadth of immune response in ducks. Attenuated Salmonella typhimurium (SL7207) was used as a carrier and adjuvant to boost the magnitude of immune response. Based on this strategy, novel DNA vaccines (SL7207-pVAX1-LC and SL7207-pVAX1-SC) were generated. Growth kinetics, genetic stabilities and relative transcription levels of the L, S and C genes introduced by these vaccine strains were measured before inoculation to guarantee safety and efficacy. The relative transcript levels of the CD4 and CD8 T genes and the antibody levels (IgY) in ducks receiving the vaccines were higher than those in single gene delivered groups. Additionally, the copy number of covalently closed circular DNA in hepatocytes after DHBV challenge also provided evidence that our fusion vaccines could enhance the protective efficiency against DHBV infection in ducks.http://www.sciencedirect.com/science/article/pii/S209531191761829Xfusion DNA genesattenuated Salmonella typhimurium (SL7207)hepatitis B virus (HBV)duck hepatitis B virus (DHBV)oral immunization |
| spellingShingle | Si-yang LIU Ren-yong JIA Qing-qing LI Dai-shen FENG Hao-yue SHEN Cui YANG Ming-shu WANG De-kang ZHU Shun CHEN Ma-feng LIU Xin-xin ZHAO Zhong-qiong YIN Bo JING An-chun CHENG Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium Journal of Integrative Agriculture fusion DNA genes attenuated Salmonella typhimurium (SL7207) hepatitis B virus (HBV) duck hepatitis B virus (DHBV) oral immunization |
| title | Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium |
| title_full | Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium |
| title_fullStr | Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium |
| title_full_unstemmed | Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium |
| title_short | Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium |
| title_sort | immunogenicity and protective efficacy of dhbv dna vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated salmonella typhimurium |
| topic | fusion DNA genes attenuated Salmonella typhimurium (SL7207) hepatitis B virus (HBV) duck hepatitis B virus (DHBV) oral immunization |
| url | http://www.sciencedirect.com/science/article/pii/S209531191761829X |
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