Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis
Colorectal cancer (CRC) is a major global health issue due to its aggressiveness and high mortality rates. While curcumin, the bioactive compound from turmeric (Curcuma longa L.), exhibits anticancer properties, its low water solubility limits therapeutic potential. The present study developed a nov...
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001148 |
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| author | Atthapan Morchang Keerakarn Somsuan Artitaya Rongjumnong Churat Weeraphan Sasivimon Pramual Theetat Ruangjaroon Daranee Chokchaichamnankit Wuttichai Jaidee Phateep Hankittichai Chanatip Pramvichai Rawiwan Charoensup Arunothai Wanta Chantragan Srisomsap Jisnuson Svasti Siripat Aluksanasuwan |
| author_facet | Atthapan Morchang Keerakarn Somsuan Artitaya Rongjumnong Churat Weeraphan Sasivimon Pramual Theetat Ruangjaroon Daranee Chokchaichamnankit Wuttichai Jaidee Phateep Hankittichai Chanatip Pramvichai Rawiwan Charoensup Arunothai Wanta Chantragan Srisomsap Jisnuson Svasti Siripat Aluksanasuwan |
| author_sort | Atthapan Morchang |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is a major global health issue due to its aggressiveness and high mortality rates. While curcumin, the bioactive compound from turmeric (Curcuma longa L.), exhibits anticancer properties, its low water solubility limits therapeutic potential. The present study developed a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin (CUR-A-Poly-β-CD) inclusion complex to enhance solubility and evaluated its anticancer potential in CRC cell lines. The complex exhibited a zeta potential of -22.9 ± 1.1 mV, a particle size of 496.8 ± 1.9 nm, a solubility of 13.6 mg/L, and a pH of 4.5. CUR-A-Poly-β-CD was non-toxic to normal colon cells (CCD-841 CoN) but significantly reduced the viability of CRC cell lines HT-29, SW-48, and SW-480, with SW-480 cells being the most responsive. CUR-A-Poly-β-CD induced apoptosis and inhibited proliferation, clonogenicity, and migration of SW-480 cells. Proteomic analysis identified 105 altered proteins in SW-480 cells treated with CUR-A-Poly-β-CD, primarily involved in cell growth and ribosome biogenesis pathways. Western blot analysis confirmed increased levels of ribosomal proteins RPL13a and RPS3. In conclusion, the CUR-A-Poly-β-CD inclusion complex demonstrates anticancer effects against CRC by modulating ribosome biogenesis. These findings suggest its potential as a therapeutic approach to improve the effectiveness of CRC treatments. |
| format | Article |
| id | doaj-art-e76c28402271471884574554604fee5d |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-e76c28402271471884574554604fee5d2025-08-20T03:20:58ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010077510.1016/j.carpta.2025.100775Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesisAtthapan Morchang0Keerakarn Somsuan1Artitaya Rongjumnong2Churat Weeraphan3Sasivimon Pramual4Theetat Ruangjaroon5Daranee Chokchaichamnankit6Wuttichai Jaidee7Phateep Hankittichai8Chanatip Pramvichai9Rawiwan Charoensup10Arunothai Wanta11Chantragan Srisomsap12Jisnuson Svasti13Siripat Aluksanasuwan14School of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand; Cancer and Immunology Research Unit (CIRU), Mae Fah Luang University, Chiang Rai 57100, ThailandSchool of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand; Cancer and Immunology Research Unit (CIRU), Mae Fah Luang University, Chiang Rai 57100, ThailandCancer and Immunology Research Unit (CIRU), Mae Fah Luang University, Chiang Rai 57100, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandMedicinal Plants Innovation Center of Mae Fah Luang University, Chiang Rai 57100, ThailandSchool of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand; Cancer and Immunology Research Unit (CIRU), Mae Fah Luang University, Chiang Rai 57100, ThailandSchool of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand; Cancer and Immunology Research Unit (CIRU), Mae Fah Luang University, Chiang Rai 57100, ThailandMedicinal Plants Innovation Center of Mae Fah Luang University, Chiang Rai 57100, Thailand; School of Integrative Medicine, Mae Fah Luang University, Chiang Rai 57100, ThailandSchool of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand; Cancer and Immunology Research Unit (CIRU), Mae Fah Luang University, Chiang Rai 57100, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandSchool of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand; Cancer and Immunology Research Unit (CIRU), Mae Fah Luang University, Chiang Rai 57100, Thailand; Corresponding author: School of Medicine, Mae Fah Luang University, 333 Moo 1, Thasud, Muang, Chiang Rai 57100, Thailand.Colorectal cancer (CRC) is a major global health issue due to its aggressiveness and high mortality rates. While curcumin, the bioactive compound from turmeric (Curcuma longa L.), exhibits anticancer properties, its low water solubility limits therapeutic potential. The present study developed a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin (CUR-A-Poly-β-CD) inclusion complex to enhance solubility and evaluated its anticancer potential in CRC cell lines. The complex exhibited a zeta potential of -22.9 ± 1.1 mV, a particle size of 496.8 ± 1.9 nm, a solubility of 13.6 mg/L, and a pH of 4.5. CUR-A-Poly-β-CD was non-toxic to normal colon cells (CCD-841 CoN) but significantly reduced the viability of CRC cell lines HT-29, SW-48, and SW-480, with SW-480 cells being the most responsive. CUR-A-Poly-β-CD induced apoptosis and inhibited proliferation, clonogenicity, and migration of SW-480 cells. Proteomic analysis identified 105 altered proteins in SW-480 cells treated with CUR-A-Poly-β-CD, primarily involved in cell growth and ribosome biogenesis pathways. Western blot analysis confirmed increased levels of ribosomal proteins RPL13a and RPS3. In conclusion, the CUR-A-Poly-β-CD inclusion complex demonstrates anticancer effects against CRC by modulating ribosome biogenesis. These findings suggest its potential as a therapeutic approach to improve the effectiveness of CRC treatments.http://www.sciencedirect.com/science/article/pii/S2666893925001148CurcuminCyclodextrinsColorectal cancerProteomicsRibosome biogenesis |
| spellingShingle | Atthapan Morchang Keerakarn Somsuan Artitaya Rongjumnong Churat Weeraphan Sasivimon Pramual Theetat Ruangjaroon Daranee Chokchaichamnankit Wuttichai Jaidee Phateep Hankittichai Chanatip Pramvichai Rawiwan Charoensup Arunothai Wanta Chantragan Srisomsap Jisnuson Svasti Siripat Aluksanasuwan Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis Carbohydrate Polymer Technologies and Applications Curcumin Cyclodextrins Colorectal cancer Proteomics Ribosome biogenesis |
| title | Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis |
| title_full | Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis |
| title_fullStr | Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis |
| title_full_unstemmed | Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis |
| title_short | Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis |
| title_sort | proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin ascorbic acid polysaccharide β cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis |
| topic | Curcumin Cyclodextrins Colorectal cancer Proteomics Ribosome biogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001148 |
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