SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix
Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles’ heel, it is their potential immunogenicity. To...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/9096059 |
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| _version_ | 1850167558785728512 |
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| author | Marta Boysen Laura Schlicksupp Ingeborg Dreher Ralf Loebbert Mario Richter |
| author_facet | Marta Boysen Laura Schlicksupp Ingeborg Dreher Ralf Loebbert Mario Richter |
| author_sort | Marta Boysen |
| collection | DOAJ |
| description | Therapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles’ heel, it is their potential immunogenicity. To better understand the impact of the size of immune complexes of mAbs on anti-drug antibody (ADA) dependent adverse reactions in Macaca fascicularis, we developed an efficient high-throughput size exclusion chromatography- (SEC-) based methodology that enables analysis of the size, size distribution, and ratio of free and ADA-complexed mAb in serum allowing for assessment of formation and clearance of circulating ADA-mAb immune complexes (CIC). |
| format | Article |
| id | doaj-art-e712935665c94f8d8aae48fc00d92636 |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e712935665c94f8d8aae48fc00d926362025-08-20T02:21:10ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/90960599096059SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal MatrixMarta Boysen0Laura Schlicksupp1Ingeborg Dreher2Ralf Loebbert3Mario Richter4AbbVie Deutschland GmbH & Co. KG, Knollstrasse 50, 67061 Ludwigshafen, GermanyAbbVie Deutschland GmbH & Co. KG, Knollstrasse 50, 67061 Ludwigshafen, GermanyAbbVie Deutschland GmbH & Co. KG, Knollstrasse 50, 67061 Ludwigshafen, GermanyAbbVie Deutschland GmbH & Co. KG, Knollstrasse 50, 67061 Ludwigshafen, GermanyAbbVie Deutschland GmbH & Co. KG, Knollstrasse 50, 67061 Ludwigshafen, GermanyTherapeutic monoclonal antibodies (mAbs) represent a milestone in pharmacological development. Their superiority is based on the combination of high specificity, low toxicity, and long half-life that characterizes biologics. If biologics have Achilles’ heel, it is their potential immunogenicity. To better understand the impact of the size of immune complexes of mAbs on anti-drug antibody (ADA) dependent adverse reactions in Macaca fascicularis, we developed an efficient high-throughput size exclusion chromatography- (SEC-) based methodology that enables analysis of the size, size distribution, and ratio of free and ADA-complexed mAb in serum allowing for assessment of formation and clearance of circulating ADA-mAb immune complexes (CIC).http://dx.doi.org/10.1155/2016/9096059 |
| spellingShingle | Marta Boysen Laura Schlicksupp Ingeborg Dreher Ralf Loebbert Mario Richter SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix Journal of Immunology Research |
| title | SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix |
| title_full | SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix |
| title_fullStr | SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix |
| title_full_unstemmed | SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix |
| title_short | SEC Based Method for Size Determination of Immune Complexes of Therapeutic Antibodies in Animal Matrix |
| title_sort | sec based method for size determination of immune complexes of therapeutic antibodies in animal matrix |
| url | http://dx.doi.org/10.1155/2016/9096059 |
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