Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients
Kinases have been implicated in the immunopathological mechanisms of Systemic Lupus Erythematosus (SLE). v-akt murine-thymoma viral-oncogene-homolog 1 (AKT1) and mitogen-activated-protein-kinase 1 (MAPK1) gene expressions in peripheral mononuclear cells from thirteen SLE patients with inactive or mi...
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| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/495934 |
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| author | Sonia Garcia-Rodriguez Jose-Luis Callejas-Rubio Norberto Ortego-Centeno Esther Zumaquero Raquel Ríos-Fernandez Salvador Arias-Santiago Pilar Navarro Jaime Sancho Mercedes Zubiaur |
| author_facet | Sonia Garcia-Rodriguez Jose-Luis Callejas-Rubio Norberto Ortego-Centeno Esther Zumaquero Raquel Ríos-Fernandez Salvador Arias-Santiago Pilar Navarro Jaime Sancho Mercedes Zubiaur |
| author_sort | Sonia Garcia-Rodriguez |
| collection | DOAJ |
| description | Kinases have been implicated in the immunopathological mechanisms of Systemic Lupus Erythematosus (SLE). v-akt murine-thymoma viral-oncogene-homolog 1 (AKT1) and mitogen-activated-protein-kinase 1 (MAPK1) gene expressions in peripheral mononuclear cells from thirteen SLE patients with inactive or mild disease were evaluated using quantitative real-time reverse-transcription polymerase-chain-reaction and analyzed whether there was any correlation with T-helper (Th) transcription factors (TF) gene expression, cytokines, and S100A8/S100A9-(Calprotectin). Age- and gender-matched thirteen healthy controls were examined. AKT1 and MAPK1 expressions were upregulated in SLE patients and correlated with Th17-(Retinoic acid-related orphan receptor (ROR)-C), T-regulatory-(Treg)-(Transforming Growth Factor Beta (TGFB)-2), and Th2-(interleukin (IL)-5)-related genes. MAPK1 expression correlated with Th1-(IL-12A, T-box TF-(T-bet)), Th2-(GATA binding protein-(GATA)-3), and IL-10 expressions. IL-10 expression was increased and correlated with plasma Tumor Necrosis Factor (TNF)-α and Th0-(IL-2), Th1-(IL-12A, T-bet), GATA3, Treg-(Forkhead/winged-helix transcription factor- (FOXP)-3), and IL-6 expressions. FOXP3 expression, FOXP3/RORC, and FOXP3/GATA3 expression ratios were increased. Plasma IL-1β, IL-12(p70), Interferon-(IFN)-γ, and IL-6 cytokines were augmented. Plasma IL-1β, IL-6, IL-2, IFN-γ, TNF-α, IL-10, and IL-13 correlated with C-reactive protein, respectively. Increased Calprotectin correlated with neutrophils. Conclusion, SLE patients presented a systemic immunoinflammatory activity, augmented AKT1 and MAPK1 expressions, proinflammatory cytokines, and Calprotectin, together with increased expression of Treg-related genes, suggesting a regulatory feedback opposing the inflammatory activity. |
| format | Article |
| id | doaj-art-e6e3d6084d3a40b894d78bbaf6da11a5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e6e3d6084d3a40b894d78bbaf6da11a52025-02-03T01:03:16ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/495934495934Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus PatientsSonia Garcia-Rodriguez0Jose-Luis Callejas-Rubio1Norberto Ortego-Centeno2Esther Zumaquero3Raquel Ríos-Fernandez4Salvador Arias-Santiago5Pilar Navarro6Jaime Sancho7Mercedes Zubiaur8Department of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, (IPBLN-CSIC), Parque Tecnológico Ciencias de la Salud, Avenida Conocimiento s/n, Armilla, 18100 Granada, SpainSystemic Autoimmune Diseases Unit, Department of Internal Medicine, San Cecilio University Hospital (SCUH), Avenida Dr. Oloriz, no. 16, Granada 18012, SpainSystemic Autoimmune Diseases Unit, Department of Internal Medicine, San Cecilio University Hospital (SCUH), Avenida Dr. Oloriz, no. 16, Granada 18012, SpainDepartment of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, (IPBLN-CSIC), Parque Tecnológico Ciencias de la Salud, Avenida Conocimiento s/n, Armilla, 18100 Granada, SpainSystemic Autoimmune Diseases Unit, Department of Internal Medicine, San Cecilio University Hospital (SCUH), Avenida Dr. Oloriz, no. 16, Granada 18012, SpainDepartment of Dermatology, SCUH, Avenida Dr. Oloriz, No. 16, Granada 18012, SpainDepartment of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, (IPBLN-CSIC), Parque Tecnológico Ciencias de la Salud, Avenida Conocimiento s/n, Armilla, 18100 Granada, SpainDepartment of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, (IPBLN-CSIC), Parque Tecnológico Ciencias de la Salud, Avenida Conocimiento s/n, Armilla, 18100 Granada, SpainDepartment of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, (IPBLN-CSIC), Parque Tecnológico Ciencias de la Salud, Avenida Conocimiento s/n, Armilla, 18100 Granada, SpainKinases have been implicated in the immunopathological mechanisms of Systemic Lupus Erythematosus (SLE). v-akt murine-thymoma viral-oncogene-homolog 1 (AKT1) and mitogen-activated-protein-kinase 1 (MAPK1) gene expressions in peripheral mononuclear cells from thirteen SLE patients with inactive or mild disease were evaluated using quantitative real-time reverse-transcription polymerase-chain-reaction and analyzed whether there was any correlation with T-helper (Th) transcription factors (TF) gene expression, cytokines, and S100A8/S100A9-(Calprotectin). Age- and gender-matched thirteen healthy controls were examined. AKT1 and MAPK1 expressions were upregulated in SLE patients and correlated with Th17-(Retinoic acid-related orphan receptor (ROR)-C), T-regulatory-(Treg)-(Transforming Growth Factor Beta (TGFB)-2), and Th2-(interleukin (IL)-5)-related genes. MAPK1 expression correlated with Th1-(IL-12A, T-box TF-(T-bet)), Th2-(GATA binding protein-(GATA)-3), and IL-10 expressions. IL-10 expression was increased and correlated with plasma Tumor Necrosis Factor (TNF)-α and Th0-(IL-2), Th1-(IL-12A, T-bet), GATA3, Treg-(Forkhead/winged-helix transcription factor- (FOXP)-3), and IL-6 expressions. FOXP3 expression, FOXP3/RORC, and FOXP3/GATA3 expression ratios were increased. Plasma IL-1β, IL-12(p70), Interferon-(IFN)-γ, and IL-6 cytokines were augmented. Plasma IL-1β, IL-6, IL-2, IFN-γ, TNF-α, IL-10, and IL-13 correlated with C-reactive protein, respectively. Increased Calprotectin correlated with neutrophils. Conclusion, SLE patients presented a systemic immunoinflammatory activity, augmented AKT1 and MAPK1 expressions, proinflammatory cytokines, and Calprotectin, together with increased expression of Treg-related genes, suggesting a regulatory feedback opposing the inflammatory activity.http://dx.doi.org/10.1155/2012/495934 |
| spellingShingle | Sonia Garcia-Rodriguez Jose-Luis Callejas-Rubio Norberto Ortego-Centeno Esther Zumaquero Raquel Ríos-Fernandez Salvador Arias-Santiago Pilar Navarro Jaime Sancho Mercedes Zubiaur Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients Mediators of Inflammation |
| title | Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients |
| title_full | Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients |
| title_fullStr | Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients |
| title_full_unstemmed | Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients |
| title_short | Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients |
| title_sort | altered akt1 and mapk1 gene expression on peripheral blood mononuclear cells and correlation with t helper transcription factors in systemic lupus erythematosus patients |
| url | http://dx.doi.org/10.1155/2012/495934 |
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